Halvaei Iman, Khalili Mohammad Ali, Razi Mohammad H, Agha-Rahimi Azam, Nottola Stefania A
Department of Clinical Embryology, Yazd Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
J Hum Reprod Sci. 2013 Jan;6(1):65-9. doi: 10.4103/0974-1208.112385.
Embryo transfer (ET) technique is one of the important factors of in vitro fertlization success. Among the different steps in ET technique, less attention has been given to embryo loading (EL). The aim was to compare the impact of two different techniques of EL on pregnancy rate in IVF/ET cycles.
In this retrospective study, 144 and 170 patients were placed in groups A and B, respectively. In Group A, the embryos were drawn directly into the ET catheter from culture microdrop under the oil. In Group B, the embryos were transferred from culture microdrop into G2 medium in center-well dish. Then, the embryos were drawn into the catheter and finally transferred into the uterus. Both groups were adjusted for other parameters based on the EL technique. The main outcome measure was pregnancy rate.
There were insignificant differences for etiology of infertility, source of sperm, type of stimulation protocol, percent of IVF or intracytoplasmic sperm injection type of ET catheter, cycles with good quality embryos and transferred embryos between two groups. The rate of both chemical and clinical pregnancy was higher in Group B compared to A, but the difference was insignificant (P = 0.09 and P = 0.1, respectively).
It seems that there is no difference in the outcome by loading the embryo from microdrop or center-well dish.
胚胎移植（ET）技术是体外受精成功的重要因素之一。在ET技术的不同步骤中，胚胎装载（EL）受到的关注较少。目的是比较两种不同的EL技术对IVF/ET周期妊娠率的影响。
在这项回顾性研究中，分别将144例和170例患者分为A组和B组。在A组中，胚胎直接从油下培养微滴中吸入ET导管。在B组中，胚胎从培养微滴转移到中心孔板的G2培养基中。然后，将胚胎吸入导管，最后转移到子宫。根据EL技术对两组的其他参数进行调整。主要观察指标为妊娠率。
两组在不孕病因、精子来源、刺激方案类型、ET导管的IVF或卵胞浆内单精子注射类型百分比、优质胚胎周期和移植胚胎方面无显著差异。B组的化学妊娠率和临床妊娠率均高于A组，但差异不显著（分别为P = 0.09和P = 0.1）。
从微滴或中心孔板装载胚胎似乎在结果上没有差异。
