Van Beneden R J, Henderson K W, Blair D G, Papas T S, Gardner H S
Duke University Marine Laboratory, Beaufort, North Carolina 28516.
Cancer Res. 1990 Sep 1;50(17 Suppl):5671S-5674S.
Neoplastic transformation of cells has often been associated with changes in cellular oncogenes. While much information has been collected in mammalian systems, relatively little is known about the molecular basis of tumor progression in lower vertebrates. For our studies, tumors were collected from feral northern pike (Esox lucius) from Ostego Lake, MI, where the local population exhibited a 15% incidence of large external lymphomas. In laboratory studies, tumors were induced under controlled conditions by known mammalian carcinogens in the Japanese medaka (Oryzias latipes), a small aquarium fish widely used in carcinogenicity studies. DNA isolated from these tumors was assayed for transforming sequences by transfection into NIH3T3 cells. DNAs from the northern pike lymphomas and the chemically induced tumors in the medaka were able to transform NIH3T3 cells and induce tumors in athymic mice. The results of our studies to date are summarized here, together with the current status of oncogene activation in other fish systems.
细胞的肿瘤转化常常与细胞癌基因的变化相关。虽然在哺乳动物系统中已经收集了大量信息，但对于低等脊椎动物肿瘤进展的分子基础却知之甚少。在我们的研究中，肿瘤取自密歇根州奥斯特戈湖的野生北梭子鱼（Esox lucius），当地种群中大型外部淋巴瘤的发病率为15%。在实验室研究中，已知的哺乳动物致癌物在日本青鳉（Oryzias latipes）（一种广泛用于致癌性研究的小型水族箱鱼类）中在受控条件下诱导肿瘤。从这些肿瘤中分离的DNA通过转染到NIH3T3细胞中检测其转化序列。来自北梭子鱼淋巴瘤和青鳉化学诱导肿瘤的DNA能够转化NIH3T3细胞并在无胸腺小鼠中诱导肿瘤。我们迄今为止的研究结果在此进行总结，并附上其他鱼类系统中癌基因激活的现状。
