Vasantha T, Kumar Awanish, Attri Pankaj, Venkatesu Pannuru, Devi R S Rama
Department of Chemistry, University of Delhi, Delhi 110 007, India.
Protein Pept Lett. 2014;21(1):15-24. doi: 10.2174/09298665113209990071.
In recent years, ionic liquids (ILs) represent a new class of biocompatible co-solvents for biomolecules. In this work, we report the apparent transfer free energies (ΔG'tr) for six amino acids (AA) from water to aqueous solutions of six ammonium based ILs (diethylammonium acetate (DEAA), diethylammonium sulfate (DEAS), triethyl ammonium acetate (TEAA), triethylammonium sulfate (TEAS), triethylammonium dihydrogen phosphate (TEAP), and trimethylammonium acetate (TMAA)) through solubility measurements, as a function of IL concentration at 298.15 K under atmospheric pressure. Salting-out effect was found for AA in aqueous IL solutions with increasing IL concentrations. In addition, we observed positive values of ΔG'tr for AA from water to ILs, indicating that the interactions between ILs and AA are unfavorable. From the obtained results, we found that the selected ammonium based ILs act as stabilizers for the structure of AA.
近年来，离子液体（ILs）代表了一类新型的用于生物分子的生物相容性共溶剂。在本工作中，我们通过溶解度测量，报告了六种氨基酸（AA）从水到六种铵基离子液体（二乙铵乙酸盐（DEAA）、二乙铵硫酸盐（DEAS）、三乙铵乙酸盐（TEAA）、三乙铵硫酸盐（TEAS）、三乙铵磷酸二氢盐（TEAP）和三甲基铵乙酸盐（TMAA））水溶液的表观转移自由能（ΔG'tr），其为298.15 K、常压下IL浓度的函数。在IL水溶液中，随着IL浓度增加，发现了AA的盐析效应。此外，我们观察到AA从水到ILs的ΔG'tr为正值，表明ILs与AA之间的相互作用是不利的。从获得的结果中，我们发现所选的铵基离子液体可作为AA结构的稳定剂。
