Institute of Preventive Medicine, National Defense Medical Center, P.O. Box 90048-700, Taipei, Taiwan, ROC.
Food Chem. 2013 Dec 1;141(3):1789-95. doi: 10.1016/j.foodchem.2013.05.002. Epub 2013 May 13.
Three sensitive and specific assays, the lateral flow assay (LFA), polymerase chain reaction assay (PCR) and reversed passive latex agglutination assay (RPLA), were selected for detection of staphylococcal enterotoxin B (SEB) from 77 clinical Staphylococcus aureus strains isolated from humans. Analytical results revealed that the LFA has almost the same detection sensitivity as that of PCR and RPLA. The concordances between the 3 assays were as follows: LFA-PCR, 92.2%; LFA-RPLA, 94.8%; and PCR-RPLA, 97.4%. For further evaluation, the LFA was used for the detection of SEB in different food matrices. The assay was able to successfully identify SEB in a wide variety of food samples at levels as low as 10 ng/mL in less than 10 min. This study proved that the LFA is an excellent tool for detection of SEB both in isolated clinical S. aureus strains and in food specimens and may prove particularly important as an early warning tool to prevent food poisoning in consumers.
三种敏感且特异的检测方法，即侧向流检测法（LFA）、聚合酶链反应检测法（PCR）和反向被动乳胶凝集检测法（RPLA），被用于从 77 株从人类身上分离出的金黄色葡萄球菌临床菌株中检测肠毒素 B（SEB）。分析结果表明，LFA 的检测灵敏度几乎与 PCR 和 RPLA 相同。三种检测方法的一致性如下：LFA-PCR，92.2%；LFA-RPLA，94.8%；PCR-RPLA，97.4%。为了进一步评估，LFA 用于检测不同食品基质中的 SEB。该检测方法能够在不到 10 分钟的时间内成功地在 10ng/mL 以下的水平检测到各种食品样本中的 SEB。这项研究证明了 LFA 是一种检测分离的临床金黄色葡萄球菌菌株和食品样本中 SEB 的极好工具，它可能被证明是一种特别重要的早期预警工具，用于预防消费者食物中毒。
