Schumacher-Perdreau F, Akatova A, Pulverer G
Institut für Medizinische Mikrobiologie und Hygiene, Universität zu Köln, Germany.
Zentralbl Bakteriol. 1995 Oct;282(4):367-71. doi: 10.1016/s0934-8840(11)80706-1.
An equal accuracy of commercially available kits, i.e., the reverse passive latex agglutination test (RPLA-Oxoid) and the Enzyme-Linked Immunosorbent Assay (SET-EIA Riedel Haen, Germany) to identify the presence of the staphylococcal enterotoxin B and the toxic shock syndrome toxin TSST-1 as well as the PCR-technique to detect the presence of the seb and tst-genes was demonstrated in 18 Staphylococcus aureus isolates. Selection of a primer pair from the etb gene sequence allowing simultaneously detection of the etb and tst-genes in S. aureus using a rapid DNA extraction method is emphasized.
在18株金黄色葡萄球菌分离株中，证明了市售试剂盒（即反向被动乳胶凝集试验（RPLA - Oxoid）和酶联免疫吸附测定（SET - EIA，德国Riedel Haen公司））在鉴定金黄色葡萄球菌肠毒素B和中毒性休克综合征毒素TSST - 1的存在方面具有相同的准确性，以及检测seb和tst基因存在的PCR技术。强调了从etb基因序列中选择一对引物，以便使用快速DNA提取方法同时检测金黄色葡萄球菌中的etb和tst基因。
