Department of Basic Health Sciences, State University of Maringá, Maringá 87020-900, Brazil.
Food Chem. 2013 Dec 1;141(3):3147-52. doi: 10.1016/j.foodchem.2013.05.144. Epub 2013 Jun 10.
The antifungal activity of ginger essential oil (GEO; Zingiber officinale Roscoe) was evaluated against Fusarium verticillioides (Saccardo) Nirenberg. The minimum inhibitory concentration (MIC) of GEO was determined by micro-broth dilution. The effects of GEO on fumonisin and ergosterol production were evaluated at concentrations of 500-5000 μg/mL in liquid medium with a 5mm diameter mycelial disc of F. verticillioides. Gas chromatography-mass spectrometry showed that the predominant components of GEO were α-zingiberene (23.9%) and citral (21.7%). GEO exhibited inhibitory activity, with a MIC of 2500 μg/mL, and 4000 and 5000 μg/mL reduced ergosterol biosynthesis by 57% and 100%, respectively. The inhibitory effect on fumonisin B1 (FB1) and fumonisin B2 (FB2) production was significant at GEO concentrations of 4000 and 2000 μg/mL, respectively. Thus, the inhibition of fungal biomass and fumonisin production was dependent on the concentration of GEO. These results suggest that GEO was able to control the growth of F. verticillioides and subsequent fumonisin production.
生姜精油（GEO；Zingiber officinale Roscoe）对串珠镰刀菌（Saccardo）Nirenberg 的抗真菌活性进行了评估。通过微量肉汤稀释法测定 GEO 的最小抑菌浓度（MIC）。在浓度为 500-5000μg/mL 的液体培养基中，用 5mm 直径的串珠镰刀菌菌丝片评估 GEO 对伏马菌素和麦角甾醇产生的影响。气相色谱-质谱法表明，GEO 的主要成分是α-姜烯（23.9%）和柠檬醛（21.7%）。GEO 表现出抑制活性，MIC 为 2500μg/mL，4000μg/mL 和 5000μg/mL 分别使麦角甾醇生物合成降低 57%和 100%。GEO 浓度分别为 4000μg/mL 和 2000μg/mL 时，对伏马菌素 B1（FB1）和伏马菌素 B2（FB2）产生的抑制作用显著。因此，真菌生物量和伏马菌素产生的抑制作用取决于 GEO 的浓度。这些结果表明，GEO 能够控制串珠镰刀菌的生长和随后的伏马菌素产生。
