为常规测定试剂次氯酸浓度而制备 2-硝基-5-硫代苯甲酸。
Preparation of 2-nitro-5-thiobenzoate for the routine determination of reagent hypochlorous acid concentrations.
机构信息
Winthrop University Hospital, Mineola, NY 11501, USA.
出版信息
Anal Biochem. 2013 Oct 15;441(2):180-1. doi: 10.1016/j.ab.2013.06.023. Epub 2013 Jul 16.
Abstract
Solutions of hypochlorous acid (HOCl) decay over time. This decay indicates the necessity for methods and reagents for the routine measurement of this oxidant. 2-Nitro-5-thiobenzoate is commonly used to measure HOCl concentrations. This article describes a method for the preparation of 2-nitro-5-thiobenzoate that is stable for at least 3 months. This method relies on the partial rather than full reduction of 5,5'-dithiobis-(2-nitrobenzoic acid) and the resulting equilibrium between the substrate and the product.
摘要
次氯酸（HOCl）溶液会随时间衰减。这表明需要有方法和试剂来对这种氧化剂进行常规测量。2-硝基-5-噻吩甲酸盐通常用于测量 HOCl 浓度。本文描述了一种至少稳定 3 个月的 2-硝基-5-噻吩甲酸盐的制备方法。该方法依赖于 5,5'-二硫代双（2-硝基苯甲酸）的部分还原而不是完全还原，以及底物和产物之间的平衡。
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