School of Chemistry, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
Spectrochim Acta A Mol Biomol Spectrosc. 2013 Nov;115:603-9. doi: 10.1016/j.saa.2013.06.068. Epub 2013 Jul 1.
We here report the CdTe quantum dot (CdTe QDs)-based sensor for probing vitamin B12 derivatives in aqueous solution. In this paper, simple and sensitive fluorescence quenching measurements has been employed. The Stern-Volmer constant (KSV), quenching rate constant (kq) and binding constant (K) were rationalized from fluorescence quenching measurement. Furthermore, the fluorescence resonance energy transfer (FRET) mechanism was discussed. This method was applicable over the concentration ranging from 1 to 14μg/mL (VB12) with correlation coefficient of 0.993. The limit of detection (LOD) of VB12 was found to be 0.15μg/mL. Moreover, the present approach opens a simple pathway for developing cost-effective, sensitive and selective QD-based fluorescence sensors/probes for biologically significant VB12 in pharmaceutical sample with mean recoveries in the range of 100-102.1%.
我们在这里报告了一种基于碲化镉量子点（CdTe QDs）的传感器，用于探测水溶液中的维生素 B12 衍生物。在本文中，我们采用了简单灵敏的荧光猝灭测量方法。从荧光猝灭测量中，我们推导出了 Stern-Volmer 常数（KSV）、猝灭速率常数（kq）和结合常数（K）。此外，我们还讨论了荧光共振能量转移（FRET）机制。该方法适用于 1 至 14μg/mL（VB12）浓度范围内，相关系数为 0.993。VB12 的检测限（LOD）为 0.15μg/mL。此外，本方法为开发具有成本效益、灵敏和选择性的基于 QD 的荧光传感器/探针，用于检测药物样品中具有生物学意义的 VB12 提供了一种简单的途径，平均回收率在 100-102.1%范围内。
