基于量子点（QDs）的荧光探针用于山奈酚的灵敏测定。
Quantum dots (QDs) based fluorescence probe for the sensitive determination of kaempferol.
作者信息
Tan Xuanping, Liu Shaopu, Shen Yizhong, He Youqiu, Yang Jidong
机构信息
School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China; School of Chemistry and Environmental Engineering, Chongqing Three Gorges University, Chongqing, Wanzhou 404000, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2014 Dec 10;133:66-72. doi: 10.1016/j.saa.2014.05.032. Epub 2014 May 23.
In this work, using the quenching of fluorescence of thioglycollic acid (TGA)-capped CdTe quantum dots (QDs), a novel method for the determination of kaempferol (KAE) has been developed. Under optimum conditions, a linear calibration plot of the quenched fluorescence intensity at 552nm against the concentration of KAE was observed in the range of 4-44μgmL(-1) with a detection limit (3σ/K) of 0.79μgmL(-1). In addition, the detailed reaction mechanism has also been proposed on the basis of electron transfer supported by ultraviolet-visible (UV-vis) absorption and fluorescence (FL) spectroscopy. The method has been applied for the determination of KAE in pharmaceutical preparations with satisfactory results. The proposed method manifested several advantages such as high sensitivity, short analysis time, low cost and ease of operation.
在本工作中，利用巯基乙酸（TGA）包覆的碲化镉量子点（QDs）荧光猝灭现象，开发了一种测定山奈酚（KAE）的新方法。在最佳条件下，观察到552nm处荧光猝灭强度与KAE浓度在4 - 44μgmL⁻¹范围内呈线性校准曲线，检测限（3σ/K）为0.79μgmL⁻¹。此外，还基于紫外可见（UV-vis）吸收光谱和荧光（FL）光谱支持的电子转移提出了详细的反应机理。该方法已应用于药物制剂中山奈酚的测定，结果令人满意。所提出的方法具有灵敏度高、分析时间短、成本低和操作简便等优点。
Zotero 插件