Department of Applied Chemistry, Cochin University of Science and Technology, Kochi, Kerala, 682022, India.
J Fluoresc. 2021 Jul;31(4):1113-1123. doi: 10.1007/s10895-021-02743-y. Epub 2021 May 8.
A heterocyclic Schiff base (MPDPI)was synthesized by the condensation reaction of 1-phenylisatin with 4,5-dimethylphenylene diamine. It was characterized by using spectroscopic methods including UV visible, Infrared, H-NMR, C-NMR and mass spectrometry. It acts as the fluorescent probe for the detection of Vitamin B12 (Vit.B12) which shows high selectivity over other species via dynamic quenching mechanism. It is also highly sensitive towards Vit.B12 with a detection limit of [Formula: see text]M and showed a linear concentration ranging from [Formula: see text] to [Formula: see text]. Effect of other coexisting species was also studied. The satisfactory results were also obtained in real samples.Since, there are only few reports on Vit.B12, development of selective fluorescent probes for Vit.B12 would be worthwhile.
一种杂环席夫碱（MPDPI）是通过 1-苯并异吲哚与 4,5-二甲基苯二胺的缩合反应合成的。它的结构特征是通过包括紫外可见光谱、红外光谱、氢核磁共振、碳核磁共振和质谱在内的光谱方法来确定。它作为荧光探针用于检测维生素 B12（Vit.B12），通过动态猝灭机制对其他物质具有高选择性。它对 Vit.B12 也具有高灵敏度，检测限为 [Formula: see text]M，并显示出从 [Formula: see text] 到 [Formula: see text] 的线性浓度范围。还研究了其他共存物质的影响。在实际样品中也得到了令人满意的结果。由于关于 Vit.B12 的报道很少，因此开发用于检测 Vit.B12 的选择性荧光探针是值得的。
