Department of Pathophysiology and Therapeutics, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
Biochem Biophys Res Commun. 2013 Aug 16;438(1):61-5. doi: 10.1016/j.bbrc.2013.07.024. Epub 2013 Jul 18.
The absorption pathway(s) of a representative food allergen, lysozyme, and the mechanisms of lysozyme absorption facilitated by non-steroidal anti-inflammatory drugs were examined by intestinal closed-loop and re-circulating perfusion methods in rats. The absorption rate of fluorescein isothiocyanate (FITC)-labeled lysozyme in the proximal intestine was higher than that for a marker of non-specific absorption, FD-10, and was suppressed by colchicine (endocytosis inhibitor). Aspirin increased the absorption of FITC-lysozyme in the proximal intestine with no effects on tissue accumulation. Diclofenac facilitated FITC-lysozyme absorption, but meloxicam and loxoprofen exerted no effects on absorption. Co-administration of misoprostol (synthetic prostaglandin-E1 analog) with aspirin significantly ameliorated the aspirin-facilitated absorption of FITC-lysozyme to the same level as that seen with controls. Thus, lysozyme absorption was mediated by endocytic and paracellular pathways in the proximal intestine, and was facilitated by aspirin and diclofenac after impairment of the paracellular pathway. Misoprostol may suppress the allergen absorption facilitated by aspirin.
采用肠袢和再循环灌流方法在大鼠中研究了代表性食物过敏原溶菌酶的吸收途径和非甾体抗炎药促进溶菌酶吸收的机制。在近端肠道中，荧光素异硫氰酸酯（FITC）标记的溶菌酶的吸收率高于非特异性吸收标志物 FD-10，并且被秋水仙碱（内吞抑制剂）抑制。阿司匹林增加了 FITC-溶菌酶在近端肠道的吸收，而对组织积累没有影响。双氯芬酸促进 FITC-溶菌酶的吸收，但美洛昔康和洛索洛芬没有影响吸收。与阿司匹林一起给予米索前列醇（合成前列腺素 E1 类似物）可显著改善阿司匹林促进的 FITC-溶菌酶吸收，使其达到与对照组相同的水平。因此，溶菌酶的吸收是通过近端肠道的内吞和细胞旁途径介导的，并且在细胞旁途径受损后，阿司匹林和双氯芬酸促进了吸收。米索前列醇可能抑制阿司匹林促进的过敏原吸收。
