Termination patterns of calcitonin gene-related peptide-immunoreactive nerve fibers in the dorsal horn of the human spinal cord.

CGRP-immunoreactive varicose nerve fibers displayed three kinds of termination patterns in the cervical, thoracic and lumbar segments of the human spinal cord. Bundles of immunoreactive fibers formed a loose network in lamina I. A homogenous band of immunoreactive fibers filled lamina II. Multiple bundles of CGRP-positive fibers coursed through the superficial laminae towards deep portions of the grey matter. In the lumbar segments, in contrast to the cervical and thoracic segments, the bundles could be followed deep into the dorsal funiculus. Bundles of varicose immunoreactive fibers were seen to twine around the dendrites of neurons located in lamina I, in the dorsal funiculus of the lumbar segments and deep in the dorsal horn (laminae III-V). The corresponding types of large and medium-sized neurons were found in silver impregnated adjacent spinal cord sections. It is suggested that neurons in the above locations preferentially receive multiple contacts from CGRP-containing nerve fibers along their extensive dendritic arborizations (CGRP-target neurons).