在Chang肝细胞中金属硫蛋白的协同和相加诱导。应激显著诱导金属硫蛋白的一种可能机制。
Synergistic and additive induction of metallothionein in Chang liver cells. A possible mechanism of marked induction of metallothionein by stress.
作者信息
Iijima Y, Fukushima T, Bhuiyan L A, Yamada T, Kosaka F, Sato J D
机构信息
Department of Anesthesiology and Resuscitology, Okayama University Medical School, Japan.
出版信息
FEBS Lett. 1990 Aug 20;269(1):218-20. doi: 10.1016/0014-5793(90)81158-k.
We have studied metallothionein (MT) induction by three primary inducers; a heavy metal, a glucocorticoid and a cytokine, and by the combinations of these inducers in the cultured cells. MT-protein was synergistically induced by either a cytokine or a heavy metal with a glucocorticoid hormone and was additively induced by the combination of a cytokine and a heavy metal, but MT-mRNA levels were not completely correlated with MT-protein levels. These results suggest that posttranscriptional regulation may be involved in the synergistic induction of MT-protein. We propose a possible mechanism in which marked MT induction by stress in vivo is dependent on the combined effect of two or more inducers, because marked MT induction is not seen by an injection of a plausible dose of either a glucocorticoid hormone or a cytokine in vivo.
我们研究了三种主要诱导剂对金属硫蛋白（MT）的诱导作用；一种重金属、一种糖皮质激素和一种细胞因子，以及这些诱导剂在培养细胞中的组合。细胞因子或重金属与糖皮质激素协同诱导MT蛋白，细胞因子和重金属的组合则以相加方式诱导MT蛋白，但MT-mRNA水平与MT蛋白水平并不完全相关。这些结果表明，转录后调控可能参与了MT蛋白的协同诱导。我们提出了一种可能的机制，即体内应激导致的显著MT诱导依赖于两种或更多诱导剂的联合作用，因为在体内注射合理剂量的糖皮质激素或细胞因子均未观察到显著的MT诱导。
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