Kobayashi S, Okada T, Kimura M
Chem Biol Interact. 1985 Nov;55(3):347-56. doi: 10.1016/s0009-2797(85)80141-1.
Metallothioneins (MTs) were induced in Chang liver cells by the metals, Zn, Cu and Cd, and the glucocorticoid hormone, dexamethasone. When 116 microM Zn, 32 microM Cu and 18 microM Cd, and 10(-7) M dexamethasone, respectively, were administered for 9 h, MTs induced by each inducer in the cells reached maximum levels. The maximum accumulation of MT level induced by dexamethasone was the lowest of the four inducers investigated; the levels induced by Zn, Cu and Cd were 4.7, 1.2 and 1.5 times of that induced by dexamethasone. When dexamethasone was added to the cells together with the heavy metals (Zn, Cu and Cd), dexamethasone had an additive effect on the maximum MT accumulations induced by heavy metals as compared to when induction was conducted using one of heavy metals alone or by dexamethasone alone. However, dexamethasone did almost not effect the metal accumulations in the cells, although the maximum MT levels induced by heavy metal increased by dexamethasone. These results suggest that the process of MT induction by heavy metals and that by dexamethasone are independent of one another. When dexamethasone was added to the cells together with a high concentration of Cu (32 microM) induced the maximum MT accumulation, Cu transport into the cells decreased by 20-40% of that into non-treated cells, which was statistically significant.
金属硫蛋白（MTs）可由锌、铜、镉等金属以及糖皮质激素地塞米松在张氏肝细胞中诱导产生。当分别给予116微摩尔锌、32微摩尔铜、18微摩尔镉以及10⁻⁷摩尔地塞米松，并作用9小时时，细胞中每种诱导剂诱导产生的MTs达到最大水平。在所研究的四种诱导剂中，地塞米松诱导产生的MT水平的最大积累量是最低的；锌、铜和镉诱导产生的水平分别是地塞米松诱导产生水平的4.7倍、1.2倍和1.5倍。当地塞米松与重金属（锌、铜和镉）一起添加到细胞中时，与单独使用一种重金属或单独使用地塞米松诱导相比，地塞米松对重金属诱导的MT最大积累量具有累加效应。然而，尽管地塞米松增加了重金属诱导的MT最大水平，但它几乎不影响细胞中的金属积累。这些结果表明，重金属诱导MT的过程与地塞米松诱导MT的过程是相互独立的。当地塞米松与高浓度的铜（32微摩尔）一起添加到细胞中诱导MT最大积累时，铜进入细胞的量比未处理细胞减少了20% - 40%，这具有统计学意义。
