Breton-Gorius J, Coquin Y, Guichard J
C R Acad Hebd Seances Acad Sci D. 1975 Apr 14;280(14):1753-6.
The neutrophils and monocytes of two patients with hereditary myeloperoxidase deficiency lacked myeloperoxidase activity as determined by light and electron microscopic cytochemical staining. Using Graham-Karnovsky media, azurophils of neutrophils were devoid of peroxidase whereas all eosinophilic and basophilic granules exhibited normal peroxidase activity. After incubation in alkaline diaminobenzidine media which stains the catalase of microperoxisomes, some small granules were seen to be strongly stained in both immature and mature neutrophils. These small granules were distinct from all other neutrophilic granules which lacked a positive reaction. Only, in the presence of cyanide or aminotriazole, peroxidatic activity was also detected in some ellipsoid azurophils. This observation suggests that these substances activated an oxidase whose nature is discussed.
通过光镜和电镜细胞化学染色测定，两名遗传性髓过氧化物酶缺乏症患者的中性粒细胞和单核细胞缺乏髓过氧化物酶活性。使用格雷厄姆-卡诺夫斯基培养基，中性粒细胞的嗜天青颗粒缺乏过氧化物酶，而所有嗜酸性和嗜碱性颗粒均表现出正常的过氧化物酶活性。在碱性二氨基联苯胺培养基中孵育后，该培养基可对微过氧化物酶体的过氧化氢酶进行染色，在未成熟和成熟的中性粒细胞中均可见一些小颗粒被强烈染色。这些小颗粒与所有其他无阳性反应的嗜中性颗粒不同。仅在存在氰化物或氨基三唑的情况下，在一些椭圆形嗜天青颗粒中也检测到过氧化物活性。这一观察结果表明，这些物质激活了一种氧化酶，其性质将进行讨论。
