Lippi U, Cappelletti P, Schinella M
Nouv Rev Fr Hematol (1978). 1984;26(1):35-8.
We studied myeloperoxidase and eosinophil peroxidase activity in healthy subjects and in four totally myeloperoxidase deficient subjects using cytochemical and biochemical methods. Cytochemical tests demonstrated a pH dependent, enzymatic differentiated affinity of myeloperoxidase and eosinophil peroxidase for the chromogens used. The eosinophil peroxidase/myeloperoxidase ratio varied from 1.5 to 4. Biochemical assays, using MBTH-AA or o-dianisidine methods, produced eosinophil peroxidase/myeloperoxidase ratios of 1.98 and 1.72.
我们使用细胞化学和生化方法，对健康受试者以及四名完全缺乏髓过氧化物酶的受试者的髓过氧化物酶和嗜酸性粒细胞过氧化物酶活性进行了研究。细胞化学测试表明，髓过氧化物酶和嗜酸性粒细胞过氧化物酶对所用色原具有pH依赖性的酶促分化亲和力。嗜酸性粒细胞过氧化物酶/髓过氧化物酶的比率在1.5至4之间变化。使用MBTH-AA或邻联茴香胺方法进行的生化分析得出嗜酸性粒细胞过氧化物酶/髓过氧化物酶的比率分别为1.98和1.72。
