• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

细胞间连接形态超微结构改变与人类食管炎相关性研究。

Relevance of ultrastructural alterations of intercellular junction morphology in inflamed human esophagus.

机构信息

Department of Life Sciences, Tzu Chi University, Hualien, Taiwan.

出版信息

J Neurogastroenterol Motil. 2013 Jul;19(3):324-31. doi: 10.5056/jnm.2013.19.3.324. Epub 2013 Jul 8.

DOI:10.5056/jnm.2013.19.3.324
PMID:23875099
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3714410/
Abstract

BACKGROUND/AIMS: Detailed characterization of the ultrastructural morphology of intercellular space in gastroesophageal reflux disease has not been fully studied. We aimed to investigate whether subtle alteration in intercellular space structure and tight junction proteins might differ among patients with gastroesophageal reflux disease.

METHODS

Esophageal biopsies at 5 cm above the gastroesophageal junction were obtained from 6 asymptomatic controls, 10 patients with reflux symptoms but without erosions, and 18 patients with erosions. The biopsies were morphologically evaluated by transmission electron microscopy, and by using immunohistochemistry for tight junction proteins (claudin-1 and claudin-2 proteins).

RESULTS

The expressions of tight junction proteins did not differ between asymptomatic controls and gastroesophageal reflux disease patients. In patients with gastroesophageal reflux disease, altered desmosomal junction morphology was only found in upper stratified squamous epithelium. Dilated intercellular space occurred only in upper stratified squamous epithelium and in patients with erosive esophagitis.

CONCLUSIONS

This study suggests that dilated intercellular space may not be uniformly present inside the esophageal mucosa and predominantly it is located in upper squamous epithelium. Presence of desmosomal junction alterations is associated with increased severity of gastroesophageal reflux disease. Besides dilated intercellular space, subtle changes in ultrastructural morphology of intercellular space allow better identification of inflamed esophageal mucosa relevant to acid reflux.

摘要

背景/目的：胃食管反流病（gastroesophageal reflux disease，GERD）细胞间隙的超微结构特征尚未得到充分研究。本研究旨在探讨 GERD 患者细胞间隙结构和紧密连接蛋白是否存在细微改变。

方法

收集食管胃结合部上 5cm 处活检组织，纳入无症状对照者 6 例、无糜烂但有反流症状者 10 例和糜烂性食管炎者 18 例。通过透射电镜观察超微结构，免疫组化检测紧密连接蛋白（claudin-1 和 claudin-2）。

结果

无症状对照者和 GERD 患者的紧密连接蛋白表达无差异。在 GERD 患者中，仅在上皮分化区可见桥粒连接改变；细胞间隙扩张仅在上皮分化区和糜烂性食管炎患者中出现。

结论

本研究提示细胞间隙扩张可能并非食管黏膜固有改变，主要位于食管上段鳞状上皮；桥粒连接改变与 GERD 严重程度相关。除细胞间隙扩张外，细胞间隙超微结构的细微改变可更好地识别与酸反流相关的炎症性食管黏膜。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd3b/3714410/f590a3e7bd69/jnm-19-324-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd3b/3714410/c29f90261477/jnm-19-324-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd3b/3714410/6779b1f0b533/jnm-19-324-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd3b/3714410/a05223fa0736/jnm-19-324-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd3b/3714410/f590a3e7bd69/jnm-19-324-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd3b/3714410/c29f90261477/jnm-19-324-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd3b/3714410/6779b1f0b533/jnm-19-324-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd3b/3714410/a05223fa0736/jnm-19-324-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd3b/3714410/f590a3e7bd69/jnm-19-324-g004.jpg

相似文献

1
Relevance of ultrastructural alterations of intercellular junction morphology in inflamed human esophagus.细胞间连接形态超微结构改变与人类食管炎相关性研究。
J Neurogastroenterol Motil. 2013 Jul;19(3):324-31. doi: 10.5056/jnm.2013.19.3.324. Epub 2013 Jul 8.
2
Dilated intercellular spaces in gastroesophageal reflux disease patients and the changes of intercellular spaces after omeprazole treatment.胃食管反流病患者细胞间隙增宽及奥美拉唑治疗后细胞间隙的变化
Chin Med J (Engl). 2008 Jul 20;121(14):1297-301.
3
Role of tight junction proteins in gastroesophageal reflux disease.紧密连接蛋白在胃食管反流病中的作用。
BMC Gastroenterol. 2012 Sep 20;12:128. doi: 10.1186/1471-230X-12-128.
4
[A study on the light microscopic measurement of intercellular space of squamous epithelium in lower-esophagus to diagnose gastroesophageal reflux disease].[一项关于通过光镜测量食管下段鳞状上皮细胞间隙以诊断胃食管反流病的研究]
Zhonghua Nei Ke Za Zhi. 2009 Mar;48(3):208-12.
5
Altered intercellular glycoconjugates and dilated intercellular spaces of esophageal epithelium in reflux disease.
Virchows Arch. 2000 Mar;436(3):207-16. doi: 10.1007/s004280050032.
6
Oesophageal mucosal intercellular space diameter and reflux pattern in childhood erosive and non-erosive reflux disease.儿童糜烂性和非糜烂性反流病的食管黏膜细胞间隙直径和反流模式。
Dig Liver Dis. 2012 Dec;44(12):981-7. doi: 10.1016/j.dld.2012.08.001. Epub 2012 Sep 10.
7
Inflammation and specialized intestinal metaplasia of cardiac mucosa is a manifestation of gastroesophageal reflux disease.贲门黏膜的炎症和特殊型肠化生是胃食管反流病的一种表现。
Ann Surg. 1997 Oct;226(4):522-30; discussion 530-2. doi: 10.1097/00000658-199710000-00013.
8
Radial distribution of dilated intercellular spaces of the esophageal squamous epithelium in patients with reflux disease exhibiting discrete endoscopic lesions.患有反流性疾病且内镜下有离散性病变患者食管鳞状上皮细胞间扩张间隙的径向分布
Dig Dis. 2004;22(2):208-12. doi: 10.1159/000080321.
9
Dilated intercellular spaces as a marker of oesophageal damage: comparative results in gastro-oesophageal reflux disease with or without bile reflux.扩张的细胞间隙作为食管损伤的标志物：有无胆汁反流的胃食管反流病的比较结果
Aliment Pharmacol Ther. 2003 Sep 1;18(5):525-32. doi: 10.1046/j.1365-2036.2003.01713.x.
10
Dilated intercellular spaces: a morphological feature of acid reflux--damaged human esophageal epithelium.细胞间间隙增宽：酸反流损伤人食管上皮的一种形态学特征。
Gastroenterology. 1996 Nov;111(5):1200-5. doi: 10.1053/gast.1996.v111.pm8898633.

引用本文的文献

1
Comparison between Conventional and Simple Measuring Methods of Mean Nocturnal Baseline Impedance in Pediatric Gastroesophageal Reflux Disease.小儿胃食管反流病夜间平均基线阻抗的传统测量方法与简易测量方法的比较
Clin Pract. 2024 Aug 27;14(5):1682-1695. doi: 10.3390/clinpract14050134.
2
Development and dysfunction of structural cells in eosinophilic esophagitis.嗜酸性粒细胞性食管炎中结构细胞的发育和功能障碍。
J Allergy Clin Immunol. 2024 Jun;153(6):1485-1499. doi: 10.1016/j.jaci.2024.04.006.
3
Proton Pump Inhibitor Therapy for Eosinophilic Esophagitis: History, Mechanisms, Efficacy, and Future Directions.

本文引用的文献

1
Is intercellular space different among layers in normal esophageal mucosa? An electron microscopic study.正常食管黏膜各层细胞间隙是否存在差异？电镜研究。
Dig Dis Sci. 2011 Dec;56(12):3492-7. doi: 10.1007/s10620-011-1804-x. Epub 2011 Jul 7.
2
Refractory heartburn: comparison of intercellular space diameter in documented GERD vs. functional heartburn.难治性烧心：有症状的胃食管反流病与功能性烧心患者细胞间隙直径的比较。
Am J Gastroenterol. 2011 May;106(5):844-50. doi: 10.1038/ajg.2010.476. Epub 2010 Dec 21.
3
Dilated intercellular space in chronic laryngitis and gastro-oesophageal reflux disease: at baseline and post-lansoprazole therapy.
质子泵抑制剂治疗嗜酸性食管炎：历史、机制、疗效及未来方向。
J Asthma Allergy. 2022 Feb 26;15:281-302. doi: 10.2147/JAA.S274524. eCollection 2022.
4
Claudin-1/4 as directly target gene of HIF-1α can feedback regulating HIF-1α by PI3K-AKT-mTOR and impact the proliferation of esophageal squamous cell though Rho GTPase and p-JNK pathway.Claudin-1/4 作为 HIF-1α 的直接靶基因，可以通过 PI3K-AKT-mTOR 途径反馈调节 HIF-1α，通过 Rho GTPase 和 p-JNK 途径影响食管鳞状细胞的增殖。
Cancer Gene Ther. 2022 Jun;29(6):665-682. doi: 10.1038/s41417-021-00328-2. Epub 2021 Jul 19.
5
Pathophysiology and treatment options for gastroesophageal reflux disease: looking beyond acid.胃食管反流病的病理生理学和治疗选择：超越酸。
Ann N Y Acad Sci. 2021 Feb;1486(1):3-14. doi: 10.1111/nyas.14501. Epub 2020 Oct 4.
6
Gastrointestinal mucosal barrier function and diseases.胃肠道黏膜屏障功能与疾病
J Gastroenterol. 2016 Aug;51(8):768-78. doi: 10.1007/s00535-016-1207-z. Epub 2016 Apr 5.
7
Estrogen Enhances Esophageal Barrier Function by Potentiating Occludin Expression.雌激素通过增强闭合蛋白的表达来增强食管屏障功能。
Dig Dis Sci. 2016 Apr;61(4):1028-38. doi: 10.1007/s10620-015-3980-6. Epub 2015 Dec 12.
8
A bama minipig model of laryngopharyngeal reflux and the change of laryngopharyngeal mucosal ultrastructure.巴马小型猪喉咽反流模型及喉咽黏膜超微结构的变化
J Neurogastroenterol Motil. 2015 Mar 30;21(2):182-8. doi: 10.5056/jnm14113.
9
Ultrastructural features of eosinophilic oesophagitis: impact of treatment on desmosomes.嗜酸性食管炎的超微结构特征：治疗对桥粒的影响
J Clin Pathol. 2015 Jan;68(1):51-6. doi: 10.1136/jclinpath-2014-202586. Epub 2014 Oct 30.
10
Role of Ultrastructural Alterations of Intercellular Junction and Tight-junction Proteins in Patients With Gastroesophageal Reflux Disease.细胞间连接和紧密连接蛋白的超微结构改变在胃食管反流病患者中的作用
J Neurogastroenterol Motil. 2013 Oct;19(4):547-8. doi: 10.5056/jnm.2013.19.4.547.
慢性喉炎和胃食管反流病中细胞间隙扩张：基线时和兰索拉唑治疗后。
Aliment Pharmacol Ther. 2010 Oct;32(7):916-24. doi: 10.1111/j.1365-2036.2010.04420.x.
4
Dilated intercellular spaces as a marker of GERD.扩张的细胞间隙作为胃食管反流病的一个标志物。
Curr Gastroenterol Rep. 2009 Jun;11(3):190-4. doi: 10.1007/s11894-009-0030-6.
5
Extraesophageal GERD.食管外反流性食管炎
Gastroenterol Clin North Am. 2008 Dec;37(4):845-58, ix. doi: 10.1016/j.gtc.2008.09.005.
6
Tight junctions/adherens junctions: basic structure and function.紧密连接/黏着连接：基本结构与功能
J Invest Dermatol. 2007 Nov;127(11):2525-32. doi: 10.1038/sj.jid.5700865.
7
Desmosome structure, composition and function.桥粒的结构、组成与功能。
Biochim Biophys Acta. 2008 Mar;1778(3):572-87. doi: 10.1016/j.bbamem.2007.07.014. Epub 2007 Aug 9.
8
Circumferential and axial distribution of esophageal mucosal damage in reflux disease.反流性疾病中食管黏膜损伤的周向和轴向分布
Dis Esophagus. 2007;20(3):232-8. doi: 10.1111/j.1442-2050.2007.00678.x.
9
Dilated intercellular spaces and acid reflux at the distal and proximal oesophagus in patients with non-erosive gastro-oesophageal reflux disease.非糜烂性胃食管反流病患者食管远端和近端的细胞间隙扩张及酸反流。
Aliment Pharmacol Ther. 2007 Mar 1;25(5):629-36. doi: 10.1111/j.1365-2036.2006.03237.x.
10
Critical role of stress in increased oesophageal mucosa permeability and dilated intercellular spaces.应激在食管黏膜通透性增加和细胞间隙增宽中的关键作用。
Gut. 2007 Sep;56(9):1191-7. doi: 10.1136/gut.2006.113688. Epub 2007 Feb 1.