Yu Zhihong, Su Ping
Department of Pharmaceutics, Wuhan Central Hospital, Wuhan, China.
Pharmazie. 2013 Jun;68(6):428-30.
The aim of this study was to explore the mechanism of domestic beta-aescin treating chronic venous insufficiency through observing its actions on the isolated canine saphenous venous tension, venous pressure, venous return and lymphatic return. The isolated canine spiral saphenous venous tension test was performed to detect the activity of the beta-aescin. Furthermore, in the condition of constant canine femoral artery perfusion kept in the extracorporeal circulation, we measured the changes of the canine femoral artery pressure, femoral artery flow and the lymphatic return flow after intravenous injection of the agent. The results showed that when beta-aescin was administrated at the dose between 5.0 x 10(-5)-5.25 x 10(-4) mol/L, it increased obviously the contractile tension of the venous to norepinephrine in a dose-dependent manner. With canine femoral artery perfusion kept constant, beta-aescin, whose doses were 50 mg and 100 mg, reinforced intently the canine femoral venous tension accelerated the rise of the venous pressure. These finding suggested that domestic betabeta-aescin extracted from Chinese Buckeye Seed had an effect on chronic venous insufficiency by strengthening the venous tension, increasing the venous pressure and promoting venous return and lymphatic return.
本研究旨在通过观察国产七叶皂苷对离体犬隐静脉张力、静脉压、静脉回流量和淋巴回流量的作用，探讨其治疗慢性静脉功能不全的机制。进行离体犬螺旋隐静脉张力试验以检测七叶皂苷的活性。此外，在体外循环维持犬股动脉恒定灌注的条件下，我们测量了静脉注射该药物后犬股动脉压力、股动脉血流量和淋巴回流量的变化。结果表明，当七叶皂苷以5.0×10(-5)-5.25×10(-4)mol/L的剂量给药时，它以剂量依赖的方式显著增加静脉对去甲肾上腺素的收缩张力。在犬股动脉灌注保持恒定的情况下，剂量为50mg和100mg的七叶皂苷强烈增强犬股静脉张力，加速静脉压的升高。这些发现表明，从中华七叶树种子中提取的国产七叶皂苷通过增强静脉张力、增加静脉压和促进静脉回流及淋巴回流，对慢性静脉功能不全有作用。
