Kirchhof B, Kirchhof U, Etscheid G
Department of Internal Medicine, St. Josef-Hospital, Engelskirchen, FRG.
Haemostasis. 1990;20(3):169-80. doi: 10.1159/000216124.
We present a standardized measurement of collagen and ADP-induced platelet aggregation with the help of a personal computer. The calculator improves the recording of measurements and the automatic calculation of parameters to define the strength of aggregation in various aggregation phases, as well as aggregation and disaggregation velocities. The dose-effect relationships between aggregants and five calculated parameters after addition of ADP and four after addition of collagen are demonstrated in the light of measurements taken in 40 healthy persons. Comparison of these measurements with those of 8 volunteers 24 h after they had taken 250 mg of acetylsalicylic acid and those of of 5 patients with typical illnesses associated with hypo- and hyperaggregability shows that analysis of the aggregation with two reagent concentrations (1, 2 and 4 micrograms/ml collagen, 1, 2 and 4 mumols/ml ADP) and the resulting values allow one to distinguish between normal, hypo- and hyperaggregable platelets.
我们借助个人计算机展示了胶原蛋白和ADP诱导的血小板聚集的标准化测量方法。该计算器改进了测量记录以及参数的自动计算，以定义各个聚集阶段的聚集强度以及聚集和解聚速度。根据对40名健康人的测量结果，展示了添加ADP后聚集剂与五个计算参数以及添加胶原蛋白后与四个计算参数之间的剂量效应关系。将这些测量结果与8名志愿者服用250毫克乙酰水杨酸24小时后的测量结果以及5名患有与血小板聚集性降低和升高相关典型疾病的患者的测量结果进行比较，结果表明，使用两种试剂浓度（1、2和4微克/毫升胶原蛋白，1、2和4微摩尔/毫升ADP）进行聚集分析以及由此得到的值能够区分正常、聚集性降低和聚集性升高的血小板。
