三碘甲状腺原氨酸的放射免疫测定：利用聚乙二醇沉淀免疫复合物。

Radioimmunoassay for triiodothyronine: use of polyethylene glycol to precipitate immune complexes.

Twomey S L, Sweat R

Clin Biochem. 1975 Jun;8(3):169-76. doi: 10.1016/s0009-9120(75)91742-7.

Abstract

A rapid radioimmunoassay (RIA) for determining serum triiodothyronine (T3) concentration has been developed. The reaction of T3 with its antibody was performed at 37 degrees C for 3 hours; precipitation of antibody bound T3 by 15% polyethylene glycol (PEG) was done at ice-bath temperatures. 2. The effects of pH on the interaction of T3 with its antibody and on the precipitation of antibody gamman globulin by PEG are discussed. 3. PEG precipitates and supernatants were characterized by immunoelectrophoresis and polyacrylamide gel electrophoresis. 4. The RIA has a between run precision of 9.3% at a mean T3 concentration of 179 ng/dl. The recovery of 50 or 100 ng/dl T3 was 95-102% and the normal range estimate was 66-215 ng/dl (N equal to 49).

摘要

已开发出一种用于测定血清三碘甲状腺原氨酸（T3）浓度的快速放射免疫分析法（RIA）。T3与其抗体的反应在37℃下进行3小时；在冰浴温度下用15%聚乙二醇（PEG）沉淀与抗体结合的T3。2. 讨论了pH对T3与其抗体相互作用以及PEG对抗体γ球蛋白沉淀的影响。3. 通过免疫电泳和聚丙烯酰胺凝胶电泳对PEG沉淀物和上清液进行表征。4. 在平均T3浓度为179 ng/dl时，该RIA的批间精密度为9.3%。50或100 ng/dl T3的回收率为95 - 102%，正常范围估计为66 - 215 ng/dl（N = 49）。