Müllenbach R, Tisborn S, Blin N
Institute of Anthropology and Human Genetics, University of Tübingen, Germany.
DNA Seq. 1994;4(4):249-52. doi: 10.3109/10425179409020848.
A series of clones from an Alu-PCR library were analysed in more detail. Characterization by Southern blot hybridization and sequencing displayed several features common to all probes generated by this approach: Short length of the PCR-products as well as the presence of homologous regions on both ends resulted in a limited feasibility for filter hybridization and a low probability of restriction length polymorphisms. In addition, a series of different short repeats at the 3'-ends of Alu-repeats and present in the generated probes offers a rich source of potential variable sites accessible by PCR.
