Department of Chemistry, Old Dominion University, Norfolk, VA 23529, USA.
Appl Spectrosc. 2013 Aug;67(8):973-84. doi: 10.1366/12-06852.
A novel Raman spectrometer is presented in a handheld format. The spectrometer utilizes a temperature-controlled, distributed Bragg reflector diode laser, which allows the instrument to operate in a sequentially shifted excitation mode to eliminate fluorescence backgrounds, fixed pattern noise, and room lights, while keeping the Raman data in true spectral space. The cost-efficient design of the instrument allows rapid acquisition of shifted excitation data with a shift time penalty of less than 2 s. The Raman data are extracted from the shifted excitation spectra using a novel algorithm that is typically three orders of magnitude faster than conventional shifted-excitation algorithms operating in spectral space. The superiority of the instrument and algorithm in terms of background removal and signal-to-noise ratio is demonstrated by comparison to FT-Raman, standard deviation spectra, shifted excitation Raman difference spectroscopy (SERDS), and conventional multiple-shift excitation methods.
本文提出了一种手持式新型拉曼光谱仪。该光谱仪采用了控温分布式布拉格反射激光二极管，使仪器能够以顺序移位激发模式运行，从而消除荧光背景、固定模式噪声和环境光，同时保持拉曼数据处于真实的光谱空间。仪器的经济型设计允许快速获取移位激发数据，移位时间惩罚小于 2 秒。拉曼数据是从移位激发光谱中提取出来的，使用一种新颖的算法，通常比在光谱空间中运行的传统移位激发算法快三个数量级。通过与傅里叶变换拉曼（FT-Raman）、标准偏差光谱、移位激发拉曼差光谱（SERDS）和传统的多次移位激发方法进行比较，证明了仪器和算法在去除背景和信噪比方面的优越性。
