Cooper John B, Marshall Sarah, Jones Richard, Abdelkader Mohamed, Wise Kent L
Appl Opt. 2014 May 20;53(15):3333-40. doi: 10.1364/AO.53.003333.
The design and operation of a novel dual-laser excitation Raman instrument is described. The use of two lasers of differing wavelengths allows for a Raman spectrum covering all fundamental modes of vibration to be collected while minimizing fluorescence and allowing for spatial compression of the spectrum on an imaging detector. The use of diode lasers with integrated distributed Bragg reflector gratings facilitates the use of an integrated thermoelectric cooler to allow collection of shifted excitation spectra for both of the lasers, further enhancing the rejection of fluorescence. An example is given, which uses seven excitation wavelengths for each laser to reconstruct the Raman spectrum of a solvent in the presence of a highly fluorescent dye by using a sequentially shifted excitation Raman reconstruction algorithm.
本文描述了一种新型双激光激发拉曼仪器的设计与操作。使用两种不同波长的激光能够在将荧光降至最低并在成像探测器上实现光谱空间压缩的同时，采集覆盖所有基本振动模式的拉曼光谱。集成分布式布拉格反射光栅的二极管激光器的使用，便于利用集成热电冷却器为两种激光采集位移激发光谱，进一步增强对荧光的抑制。文中给出了一个示例，该示例通过使用顺序位移激发拉曼重建算法，针对每种激光使用七个激发波长，在存在高荧光染料的情况下重建溶剂的拉曼光谱。
