Department of Plastic Surgery, Faculty of Medicine, Oita University, Oita, Japan.
Department of Dermatology, Faculty of Medicine, Oita University, Oita, Japan.
J Invest Dermatol. 2014 Jan;134(1):256-263. doi: 10.1038/jid.2013.305. Epub 2013 Jul 22.
Dermatopontin (DP) is a small extracellular matrix component in the dermis. Fibrin is a major component of a provisional matrix that is formed just after wounding. Previously, we found that DP was present in the provisional matrix, and it interacted with fibrin. Here, we examined the role of DP on fibrin function. DP interacted with both the fibrin monomer and fibrils, and was incorporated into the fibrils during fibrin formation. A DP sequence, PHGQVVVAVRS, was identified as a fibrin-binding site, and a globular D domain of fibrin was the binding site for DP. DP accelerated fibrin fibril formation into structurally modified fibrils. Fibrin fibrils formed in the presence of DP enhanced both endothelial cell attachment and cell spreading. The attached cells developed a more organized cytoskeleton when compared with those that attached to fibrin fibrils only. The main receptor for cell adhesion was identified as αvβ3 integrin, and a cooperating receptor was a β1-containing integrin species, probably α5β1 integrin. These results indicate that DP can modify certain biological functions of fibrin, and thus a another function of this extracellular matrix protein was revealed. In addition, the fibrin-DP complex might become useful for developing an improved artificial matrix for improving wound healing.
真皮酮（DP）是真皮中一种小型细胞外基质成分。纤维蛋白是在创伤后立即形成的临时基质的主要成分。以前，我们发现 DP 存在于临时基质中，并且它与纤维蛋白相互作用。在这里，我们研究了 DP 对纤维蛋白功能的作用。DP 与纤维蛋白单体和纤维丝相互作用，并在纤维蛋白形成过程中被整合到纤维丝中。鉴定出 DP 的一个 PHGQVVVAVRS 序列是纤维蛋白结合位点，纤维蛋白的一个球形 D 结构域是 DP 的结合位点。DP 加速纤维蛋白纤维丝形成结构修饰的纤维丝。在 DP 存在下形成的纤维蛋白纤维丝增强了内皮细胞的附着和细胞扩展。与仅附着于纤维蛋白纤维丝的细胞相比，附着的细胞形成更有组织的细胞骨架。细胞黏附的主要受体被鉴定为 αvβ3 整合素，协同受体是含有 β1 的整合素种类，可能是 α5β1 整合素。这些结果表明 DP 可以修饰纤维蛋白的某些生物学功能，从而揭示了这种细胞外基质蛋白的另一个功能。此外，纤维蛋白-DP 复合物可能成为用于开发改善伤口愈合的改良人工基质的有用物质。
