Slutzky-Goldberg Iris, Hanut Aiham, Matalon Shlomo, Baev Valery, Slutzky Hagay
Department of Endodontics, Hadassah School of Dental Medicine, Hebrew University, Jerusalem, Israel.
J Endod. 2013 Aug;39(8):980-3. doi: 10.1016/j.joen.2013.04.040. Epub 2013 Jun 12.
Sodium hypochlorite (NaOCl) and calcium hydroxide (Ca[OH]2) have tissue dissolution capacity. The aim of this study was to evaluate the potential effect of dentin on their tissue dissolution capacity in a novel dentin model.
Dentin models were prepared from 25 freshly extracted human molar teeth; the crowns were separated from the roots, and a rectangular inner shape was prepared. Pulp tissue samples adjusted to similar weights of 6.5 ± 0.2 mg were randomly divided into 6 groups: NaOCl groups in test tubes or dentin models for 1 hour, Ca(OH)2 groups in test tubes or dentin models for 1 week, and control groups saline in test tubes or dentin models for 1 week. The final weights after the experimental period were checked and compared with the initial weights. The differences were statistically analyzed.
The tissue dissolution capacity of Ca(OH)2 was affected by the presence of dentin. Similarly, NaOCl lost its effect on the pulp tissue after incubation in dentin. Comparison between all test groups showed highly significant differences (P < .001).
Dentin has a detrimental effect on the ability of NaOCl and Ca(OH)2 to dissolve pulp tissue. The dentin model appears to be an efficient tool for the study of interactions between local endodontic medicaments, dentin, and pulp tissue.
次氯酸钠（NaOCl）和氢氧化钙（Ca[OH]₂）具有组织溶解能力。本研究的目的是在一种新型牙本质模型中评估牙本质对它们组织溶解能力的潜在影响。
从25颗新鲜拔除的人类磨牙制备牙本质模型；将牙冠与牙根分离，并制备出矩形内部形状。将调整至相似重量6.5±0.2毫克的牙髓组织样本随机分为6组：在试管或牙本质模型中放置1小时的NaOCl组，在试管或牙本质模型中放置1周的Ca(OH)₂组，以及在试管或牙本质模型中放置1周的对照组生理盐水。检查实验期后的最终重量并与初始重量进行比较。对差异进行统计学分析。
Ca(OH)₂的组织溶解能力受牙本质存在的影响。同样，NaOCl在牙本质中孵育后对牙髓组织失去了作用。所有测试组之间的比较显示出高度显著差异（P <.001）。
牙本质对NaOCl和Ca(OH)₂溶解牙髓组织能力有不利影响。牙本质模型似乎是研究局部牙髓药物、牙本质和牙髓组织之间相互作用的有效工具。
