Rossi-Fedele Giampiero, Steier Liviu, Dogramaci Esma Jane, Canullo Luigi, Steier Gabriela, de Figueiredo Jose Antonio Poli
Postgraduate Dental Education Unit, Institute of Clinical Education, Warwick Medical School, University of Warwick, Coventry, UK.
Aust Endod J. 2013 Aug;39(2):57-61. doi: 10.1111/j.1747-4477.2010.00287.x. Epub 2010 Nov 5.
The aim of this study was to evaluate the bovine pulp tissue dissolution ability of HealOzone, Aquatine Alpha Electrolyte® and 0.5% sodium hypochlorite, used alone or in combination. Thirty bovine pulp fragments were weighed, divided into six groups and placed individually in Eppendorf tubes containing the tested solution until total dissolution occurred. The groups were: G1: saline (negative control), G2: Aquatine Alpha Electrolyte®, G3: 0.5% NaOCl (positive control), G4: Saline + HealOzone, G5: 0.5% NaOCl + HealOzone, G6: Aquatine Alpha Electrolyte® + HealOzone. HealOzone was activated for 2 min with a #6 cup covering the test tube opening on a fixed platform. Two blinded observers using 2× loupes magnification assessed the samples continuously for the first 2 h, and then every hour for the next 8 h. Dissolution speed was calculated by dividing pulp weight by dissolution time (mg min(-1) ). G3 (NaOCl) and G5 (NaOCl + HealOzone) dissolved the pulp tissue completely. The mean dissolution speed for G3 was 0.396 mg min(-1) (SD 0.032) and for G5 was 0.775 mg min(-1) (SD 0.2). Student's t-test showed that G5 dissolved bovine pulp tissue faster than G3 (P = 0.01). Only groups containing sodium hypochlorite dissolved pulp tissue, whilst HealOzone enhanced speed of dissolution.
本研究的目的是评估单独使用或联合使用HealOzone、Aquatine Alpha Electrolyte®和0.5%次氯酸钠时对牛牙髓组织的溶解能力。称取30个牛牙髓碎片，分为6组，分别置于含有受试溶液的Eppendorf管中，直至完全溶解。分组如下：G1：生理盐水（阴性对照），G2：Aquatine Alpha Electrolyte®，G3：0.5% NaOCl（阳性对照），G4：生理盐水+HealOzone，G5：0.5% NaOCl+HealOzone，G6：Aquatine Alpha Electrolyte®+HealOzone。使用#6杯覆盖固定平台上的试管开口，将HealOzone激活2分钟。两名不知情的观察者使用2倍放大镜，在最初2小时内连续评估样本，随后8小时内每小时评估一次。通过将牙髓重量除以溶解时间（mg min(-1)）计算溶解速度。G3（NaOCl）和G5（NaOCl+HealOzone）使牙髓组织完全溶解。G3的平均溶解速度为0.396 mg min(-1)（标准差0.032），G5为0.775 mg min(-1)（标准差0.2）。学生t检验显示，G5溶解牛牙髓组织的速度比G3快（P = 0.01）。只有含次氯酸钠的组溶解了牙髓组织，而HealOzone提高了溶解速度。
