Siqueira José F, Alves Flávio R F, Versiani Marco A, Rôças Isabela N, Almeida Bernardo M, Neves Mônica A S, Sousa-Neto Manoel D
Department of Endodontics, Faculty of Dentistry, Estácio de Sá University, Rio de Janeiro, Rio de Janeiro, Brazil.
J Endod. 2013 Aug;39(8):1044-50. doi: 10.1016/j.joen.2013.04.034. Epub 2013 Jun 5.
This ex vivo study evaluated the disinfecting and shaping ability of 3 protocols used in the preparation of mesial root canals of mandibular molars by means of correlative bacteriologic and micro-computed tomographic (μμCT) analysis.
The mesial canals of extracted mandibular molars were contaminated with Enterococcus faecalis for 30 days and assigned to 3 groups based on their anatomic configuration as determined by μCT analysis according to the preparation technique (Self-Adjusting File [ReDent-Nova, Ra'anana, Israel], Reciproc [VDW, Munich, Germany], and Twisted File [SybronEndo, Orange, CA]). In all groups, 2.5% NaOCl was the irrigant. Canal samples were taken before (S1) and after instrumentation (S2), and bacterial quantification was performed using culture. Next, mesial roots were subjected to additional μCT analysis in order to evaluate shaping of the canals.
All instrumentation protocols promoted a highly significant intracanal bacterial reduction (P < .001). Intergroup quantitative and qualitative comparisons disclosed no significant differences between groups (P > .05). As for shaping, no statistical difference was observed between the techniques regarding the mean percentage of volume increase, the surface area increase, the unprepared surface area, and the relative unprepared surface area (P > .05). Correlative analysis showed no statistically significant relationship between bacterial reduction and the mean percentage increase of the analyzed parameters (P > .05).
The 3 instrumentation systems have similar disinfecting and shaping performance in the preparation of mesial canals of mandibular molars.
本体外研究通过相关细菌学和显微计算机断层扫描（μCT）分析，评估了用于下颌磨牙近中根管预备的3种方案的消毒和成形能力。
将拔除的下颌磨牙近中根管用粪肠球菌污染30天，根据μCT分析确定的解剖结构，按照预备技术（自调式锉[ReDent-Nova，以色列拉阿纳纳]、往复锉[VDW，德国慕尼黑]和扭转锉[SybronEndo，美国加利福尼亚州奥兰治]）将其分为3组。所有组均使用2.5%次氯酸钠作为冲洗液。在器械预备前（S1）和预备后（S2）采集根管样本，采用培养法进行细菌定量分析。接下来，对近中根进行额外的μCT分析，以评估根管的成形情况。
所有器械预备方案均使根管内细菌数量显著减少（P <.001）。组间定量和定性比较显示，各组之间无显著差异（P >.05）。关于根管成形，在体积增加的平均百分比、表面积增加、未预备表面积和相对未预备表面积方面，各技术之间未观察到统计学差异（P >.05）。相关性分析显示，细菌减少与分析参数的平均百分比增加之间无统计学显著关系（P >.05）。
在制备下颌磨牙近中根管时，这3种器械系统具有相似的消毒和成形性能。
