Department of Neurology, Justus-Liebig-University Giessen, Klinikstrasse 33, 35392 Giessen, Germany.
J Neurosci Methods. 2013 Sep 30;219(1):113-23. doi: 10.1016/j.jneumeth.2013.07.010. Epub 2013 Jul 20.
Flat-panel volumetric computed tomography (fpVCT) is a non-invasive approach to three-dimensional small animal imaging. The capability of volumetric scanning and a high resolution in time and space enables whole organ perfusion studies. We aimed to assess feasibility and validity of fpVCT in cerebral perfusion measurement with impaired hemodynamics by evaluation of three well-established rat stroke models for temporary and permanent middle cerebral artery occlusion (MCAO). Male Wistar rats were randomly assigned to temporary (group I: suture model) and permanent (group II: suture model; III: macrosphere model) MCAO and to a control group. Perfusion scans with respect to cerebral blood flow (CBF) and volume (CBV) were performed 24h post intervention by fpVCT, using a Gantry rotation time of 1s and a total scanning time of 30s. Postmortem analysis included infarct-size calculation by 2,3,5-triphenyltetrazolium chloride (TTC) staining. Infarct volumes did not differ significantly throughout intervention groups. After permanent MCAO, CBF significantly decreased in subcortical regions to 78.2% (group II, p=0.005) and 79.9% (group III, p=0.012) and in total hemisphere to 77.4% (group II, p=0.010) and 82.0% (group III, p=0.049). CBF was less impaired with temporary vessel occlusion. CBV measurement revealed no significant differences. Results demonstrate feasibility of cerebral perfusion quantification in rats with the fpVCT, which can be a useful tool for non-invasive dynamic imaging of cerebral perfusion in rodent stroke models. In addition to methodological advantages, CBF data confirm the macrosphere model as a useful alternative to the suture model for permanent experimental MCAO.
平板容积计算机断层扫描（fpVCT）是一种非侵入性的三维小动物成像方法。其容积扫描能力和时间与空间分辨率高，可实现全器官灌注研究。我们旨在通过评估三种成熟的大鼠短暂性和永久性大脑中动脉闭塞（MCAO）模型，评估 fpVCT 在血流动力学受损的脑灌注测量中的可行性和有效性。雄性 Wistar 大鼠随机分为短暂性（I 组：缝线模型）和永久性（II 组：缝线模型；III 组：微球模型）MCAO 组和对照组。干预后 24 小时，通过 fpVCT 进行灌注扫描，以评估脑血流量（CBF）和体积（CBV），Gantry 旋转时间为 1s，总扫描时间为 30s。死后分析包括 2,3,5-三苯基氯化四氮唑（TTC）染色计算梗死面积。整个干预组的梗死体积没有显著差异。永久性 MCAO 后，皮质下区域的 CBF 显著降低至 78.2%（II 组，p=0.005）和 79.9%（III 组，p=0.012），总半球的 CBF 降低至 77.4%（II 组，p=0.010）和 82.0%（III 组，p=0.049）。暂时性血管闭塞时 CBF 受损较轻。CBV 测量无显著差异。结果表明，fpVCT 可用于定量测量大鼠脑灌注，这可能是啮齿动物卒中模型中脑灌注非侵入性动态成像的有用工具。除了方法学上的优势外，CBF 数据还证实微球模型是永久性实验性 MCAO 的缝线模型的有用替代模型。
