Donatelli I, Takhonova A M, Klimov A I, Ghendon Y Z, Oxford J S
Division of Virology, Istituto Superiore di Sanita, Rome, Italy.
J Med Virol. 1990 Jun;31(2):112-9. doi: 10.1002/jmv.1890310208.
Eighteen influenza A (H3N2) viruses were isolated during a single outbreak in a closed community of Carmelite nuns. Serological analysis of the virus haemagglutinin (HAs), using a panel of monoclonal antibodies, demonstrated antigenic microheterogeneity. In contrast, no significant biochemical differences were detected in viral genes by RNA:RNA hybridisation or in structural or nonstructural polypeptides analysed by high-resolution polyacrylamide gel electrophoresis (PAGE) or by limited proteolysis followed by PAGE. Influenza A (H3N2) viruses isolated in the vicinity of the convent were distinguishable from each other and from the epidemic viruses isolated in the convent both antigenically and biochemically.
在加尔默罗会修女的一个封闭社区发生的一次单一疫情期间，分离出了18株甲型H3N2流感病毒。使用一组单克隆抗体对病毒血凝素（HA）进行血清学分析，显示出抗原微异质性。相比之下，通过RNA:RNA杂交在病毒基因中未检测到明显的生化差异，通过高分辨率聚丙烯酰胺凝胶电泳（PAGE）或有限蛋白酶解后再进行PAGE分析的结构或非结构多肽中也未检测到明显的生化差异。在修道院附近分离出的甲型H3N2流感病毒在抗原性和生化特性上彼此之间以及与在修道院分离出的流行病毒都有所不同。
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