Iamnikova S S, Duglas A R, Skeĭl D D, Zakstel'skaia L Ia, Iakhno M A
Vopr Virusol. 1980 Sep-Oct(5):555-8.
Monoclonal antibodies for the A/England/864/75 strain hemagglutinin were used for antigenic analysis of influenza A (H3N2) viruses isolated in the USSR in December 1979--January 1980. The results of studies with 5 different monoclones were compared with those obtained with postinfection ferret sera. It was shown that several variants of epidemic strain could circulate during one epidemic season. The epidemic in the USSR in December, 1979--January 1980 was caused by influenza viruses H3N2 similar to A/Texas/1/77 as well as further driftstrains. Four epidemic strains were circulating which differed by the composition of antigenic determinants of hemagglutinin. Members of the first group shared 4 out of 5 determinants with A/England/864/75 strains, the second group differed by two monoclones and was identical with the A/Texas/1/77 strain. The third group differed in 3 determinants and was similar to the A/Bangkok/1/79 strain, the fourth group was related to the A/England/864/75 strain only by one out of five clones. The method of monoclonal antibodies was shown to be highly sensitive and could be satisfactorily used for subtle antigenic analysis of influenza viruses.
用针对A/英格兰/864/75株血凝素的单克隆抗体对1979年12月至1980年1月在前苏联分离的甲型流感（H3N2）病毒进行抗原分析。将5种不同单克隆抗体的研究结果与感染后雪貂血清的研究结果进行比较。结果表明，在一个流行季节可能有几种流行毒株的变体同时传播。1979年12月至1980年1月在前苏联发生的疫情是由类似于A/得克萨斯/1/77的H3N2流感病毒以及进一步的漂移株引起的。有4种流行毒株在传播，它们的血凝素抗原决定簇组成不同。第一组毒株与A/英格兰/864/75株共有5个决定簇中的4个，第二组有两个单克隆抗体不同，与A/得克萨斯/1/77株相同。第三组有3个决定簇不同，与A/曼谷/1/79株相似，第四组仅与A/英格兰/864/75株的5个克隆中的1个有关。结果表明，单克隆抗体方法高度灵敏，可满意地用于流感病毒的精细抗原分析。
