Griffiths D E, Houghton R L, Lancashire W E, Meadows P A
Eur J Biochem. 1975 Feb 21;51(2):393-402. doi: 10.1111/j.1432-1033.1975.tb03939.x.
Venturicidin is a specific inhibitor of aerobic growth of yeast and has no effect on fermentative growth, a result which is consistent with its known mode of action on mitochondrial oxidative phosphorylation. Venturicidin-resistant mutants of Saccharomyces cerevisiae have been isolated and form two general classes: class 1, nuclear mutants which are resistant to a variety of mitochondrial inhibitors and uncouplers, and class 2, mitochondrial mutants of phenotype VENR OLYR and VENR TETR in vivo. VENR OLYR mutants show a high degree of resistance to venturicidin and oligomycin at the whole cell and mitochondrial ATPase level but, in contrast, no resistance at the mitochondrial level is observed with VENR TETR mutants. Venturicidin resistance/sensitivity can be correlated with two binding sites on mitochondrial ATPase, one of which is common to the oligomycin binding site and the other is common to the triethyl tin binding site. Biochemical genetic studies indicate that two mitochondrial genes specify venturicidin resistance/sensitivity and that the mitochondrial gene products are components of the mitochondrial ATPase complex.
抗霉素A是酵母有氧生长的特异性抑制剂，对发酵生长无影响，这一结果与其已知的对线粒体氧化磷酸化的作用方式一致。已经分离出酿酒酵母的抗抗霉素A突变体，它们分为两大类：第1类是对多种线粒体抑制剂和解偶联剂具有抗性的核突变体，第2类是体内表型为VENR OLYR和VENR TETR的线粒体突变体。VENR OLYR突变体在全细胞和线粒体ATP酶水平上对抗霉素A和寡霉素表现出高度抗性，但相比之下，VENR TETR突变体在线粒体水平上未观察到抗性。抗霉素A抗性/敏感性可与线粒体ATP酶上的两个结合位点相关，其中一个与寡霉素结合位点相同，另一个与三乙锡结合位点相同。生化遗传学研究表明，两个线粒体基因决定抗霉素A抗性/敏感性，并且线粒体基因产物是线粒体ATP酶复合体的组成部分。
