Thibault L, Nagai K, Hashida A, Yanaihara N, Nakagawa H
Division of Protein Metabolism, Osaka University, Japan.
Physiol Behav. 1990 May;47(5):911-5. doi: 10.1016/0031-9384(90)90019-z.
Diet self-selection from carbohydrate, protein and lipid offered simultaneously was studied in adult male rats infused for 8 days with pyroglutamyl-CCK-8 (pGlu-CCK-8) (0.8 pmol/hr), a derivative of the COOH-terminal octapeptide of cholecystokinin (CCK), or vehicle bilaterally into the ventromedial hypothalamus (VMH). Infusion with pGlu-CCK-8 markedly decreased total daily energy intake mainly due to a suppressed carbohydrate intake. Both pGlu-CCK-8 and vehicle infusions caused a nonspecific decrease in protein intake, but the vehicle infusion caused a compensatory increase in lipid intake as well as carbohydrate intake, so their total energy intake was well maintained. These results suggest that the process of infusion into the VMH elicited itself a suppressive effect on protein intake, but that pGlu-CCK-8 infusion elicited a more specific suppressive effect on carbohydrate intake and, to a lesser extent, on lipid intake. These results also support the prediction that centrally administered CCK may suppress food intake and show in addition that such a suppression occurs selectively. The similarities and the differences in the patterns of macronutrient selection produced by vehicle of pGlu-CCK-8 infusions into the VMH provide further evidence of the unique functions of VMH and CCK in the feeding process.
研究了成年雄性大鼠在双侧脑室腹内侧下丘脑（VMH）注入焦谷氨酰 - CCK - 8（pGlu - CCK - 8）（0.8 pmol/小时）（胆囊收缩素（CCK）羧基末端八肽的衍生物）或载体8天期间，同时从提供的碳水化合物、蛋白质和脂质中进行饮食自我选择的情况。注入pGlu - CCK - 8显著降低了每日总能量摄入，主要是由于碳水化合物摄入量受到抑制。pGlu - CCK - 8和载体注入均导致蛋白质摄入量非特异性下降，但载体注入导致脂质摄入量以及碳水化合物摄入量出现代偿性增加，因此它们的总能量摄入得到了良好维持。这些结果表明，向VMH注入的过程本身对蛋白质摄入产生了抑制作用，但pGlu - CCK - 8注入对碳水化合物摄入产生了更特异性的抑制作用，对脂质摄入的抑制作用较小。这些结果还支持了中枢给予CCK可能抑制食物摄入的预测，此外还表明这种抑制具有选择性。向VMH注入pGlu - CCK - 8的载体所产生的常量营养素选择模式中的异同，为VMH和CCK在进食过程中的独特功能提供了进一步的证据。
