Department of Chemical Engineering, Alagappa College of Technology Campus, Anna University, Chennai 600 025, Tamil Nadu, India.
Department of Chemical Engineering, Alagappa College of Technology Campus, Anna University, Chennai 600 025, Tamil Nadu, India.
J Biosci Bioeng. 2014 Jan;117(1):86-91. doi: 10.1016/j.jbiosc.2013.06.018. Epub 2013 Jul 24.
This work emphasizes the potential of the isolated Bacillus sp. lipase for the production of fatty acid methyl ester by the direct transesterification of Oedogonium sp. of macroalgae. Dimethyl carbonate was used as the extraction solvent and also as the reactant. The effect of solvent/algae ratio, water addition, catalyst, temperature, stirring and time on the direct transesterification was studied. The highest fatty acid methyl ester yield obtained under optimum conditions (5 g Oedogonium sp. powder, 7.5 ml of solvent (dimethyl carbonate)/g of algae, 8% catalyst (%wt/wt of oil), distilled water 1% (wt/wt of algae), 36 h, 55°C and 180 rpm) was 82%. Final product was subjected to thermogravimetric analysis and (1)H NMR analysis. The results showed that the isolated enzyme has good potential in catalyzing the direct transesterification of algae, and the dimethyl carbonate did not affect the activity of the isolated lipase.
这项工作强调了分离的芽孢杆菌脂肪酶在通过大型藻类 Oedogonium sp. 的直接酯交换生产脂肪酸甲酯方面的潜力。碳酸二甲酯被用作提取溶剂和反应物。研究了溶剂/藻类比、加水、催化剂、温度、搅拌和时间对直接酯交换的影响。在最佳条件下（5 g Oedogonium sp. 粉末、7.5 ml 溶剂（碳酸二甲酯）/g 藻类、8%的催化剂（油重的%wt/wt）、蒸馏水中 1%（藻类的 wt/wt）、36 h、55°C 和 180 rpm）下获得的最高脂肪酸甲酯产率为 82%。最终产物进行了热重分析和（1）H NMR 分析。结果表明，分离的酶在催化藻类的直接酯交换方面具有良好的潜力，并且碳酸二甲酯不影响分离脂肪酶的活性。
