School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China.
College of Food Science, Southwest University, Chongqing 400715, China.
Bioresour Technol. 2017 Jul;235:18-24. doi: 10.1016/j.biortech.2017.03.086. Epub 2017 Mar 18.
This study reported a novel immobilized MAS1 lipase from marine Streptomyces sp. strain W007 for synthesizing high-yield biodiesel from waste cooking oils (WCO) with one-step addition of methanol in a solvent-free system. Immobilized MAS1 lipase was selected for the transesterification reactions with one-step addition of methanol due to its much more higher biodiesel yield (89.50%) when compared with the other three commercial immobilized lipases (<10%). The highest biodiesel yield (95.45%) was acquired with one-step addition of methanol under the optimized conditions. Moreover, it was observed that immobilized MAS1 lipase retained approximately 70% of its initial activity after being used for four batch cycles. Finally, the obtained biodiesel was further characterized using FT-IR, H and C NMR spectroscopy. These findings indicated that immobilized MAS1 lipase is a promising catalyst for biodiesel production from WCO with one-step addition of methanol under high methanol concentration.
本研究报道了一种新型固定化 MAS1 脂肪酶，来自海洋链霉菌 W007 菌株，用于在无溶剂体系中通过一步添加甲醇从废食用油 (WCO) 合成高产率的生物柴油。由于与其他三种商业固定化脂肪酶 (<10%) 相比，固定化 MAS1 脂肪酶在一步添加甲醇时具有更高的生物柴油产率 (89.50%)，因此选择固定化 MAS1 脂肪酶用于酯交换反应。在优化条件下，通过一步添加甲醇获得了最高的生物柴油产率 (95.45%)。此外，观察到固定化 MAS1 脂肪酶在使用四个批次循环后保留了其初始活性的约 70%。最后，使用傅里叶变换红外光谱 (FT-IR)、H 和 C 核磁共振光谱对所得生物柴油进行了进一步表征。这些发现表明，固定化 MAS1 脂肪酶是在高甲醇浓度下通过一步添加甲醇从废食用油生产生物柴油的有前途的催化剂。
