Analytical & Environmental Science Division, Department of Forensic & Analytical Sciences, King's College London, 150 Stamford Street, London SE1 9NH, United Kingdom.
Forensic Sci Int. 2013 Sep 10;231(1-3):150-6. doi: 10.1016/j.forsciint.2013.04.017. Epub 2013 May 21.
The ability to link criminal activity and identity using validated analytical approaches can be of great value to forensic scientists. Herein, the factors affecting the recovery and detection of inorganic and organic energetic material residues within chemically or physically enhanced fingermarks on paper and glass substrates are presented using micro-bore anion exchange chromatography with suppressed conductivity detection. Fingermarks on both surfaces were enhanced using aluminium powder or ninhydrin after spiking with model test mixtures or through contact with black-powder substitutes. A quantitative study of the effects of environmental/method interferences, the sweat matrix, the surface and the enhancement technique on the relative anion recovery of forensically relevant species is presented. It is shown that the analytical method could detect target analytes at the nanogram level even within excesses of enhancement reagents and their reaction products when using solid phase extraction and/or microfiltration. To our knowledge, this work demonstrates for the first time that ion chromatography can detect anions in energetic materials within fingermarks on two very different surfaces, after operational enhancement techniques commonly used by forensic scientists and police have been applied.
利用经过验证的分析方法将犯罪活动与身份联系起来的能力对法医学家来说具有重要价值。本文采用微径阴离子交换色谱-抑制电导检测法，研究了化学或物理增强后的纸张和玻璃基底上指印内无机和有机含能残留物的回收和检测的影响因素。在对模型测试混合物进行点刺或与黑色火药替代品接触后，使用铝粉或茚三酮对两种表面的指印进行增强。本文对环境/方法干扰、汗液基质、表面和增强技术对相关物种相对阴离子回收率的影响进行了定量研究。结果表明，即使在使用固相萃取和/或微滤时，分析方法也可以在增强试剂及其反应产物过剩的情况下，以纳克级的水平检测到目标分析物。据我们所知，这项工作首次证明，离子色谱法可以在经过法医学家和警察常用的操作增强技术处理后，在两种非常不同的表面上的指印内检测到含能材料中的阴离子。
