Centre for Functional Ecology, Department of Life Sciences, University of Coimbra, Coimbra 3001-401, Portugal; Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
Chemosphere. 2013 Oct;93(7):1386-92. doi: 10.1016/j.chemosphere.2013.06.077. Epub 2013 Jul 23.
The aim of this study was to investigate the effects of metal mobilizing plant-growth beneficial bacterium Phyllobacterium myrsinacearum RC6b on plant growth and Cd, Zn and Pb uptake by Sedum plumbizincicola under laboratory conditions. Among a collection of metal-resistant bacteria, P. myrsinacearum RC6b was specifically chosen as a most favorable metal mobilizer based on its capability of mobilizing high concentrations of Cd, Zn and Pb in soils. P. myrsinacearum RC6b exhibited a high degree of resistance to Cd (350 mg L(-1)), Zn (1000 mg L(-1)) and Pb (1200 mg L(-1)). Furthermore, P. myrsinacearum RC6b showed multiple plant growth beneficial features including the production of 1-aminocyclopropane-1-carboxylic acid deaminase, indole-3-acetic acid, siderophore and solubilization of insoluble phosphate. Inoculation of P. myrsinacearum RC6b significantly increased S. plumbizincicola growth and organ metal concentrations except Pb, which concentration was lower in root and stem of inoculated plants. The results suggest that the metal mobilizing P. myrsinacearum RC6b could be used as an effective inoculant for the improvement of phytoremediation in multi-metal polluted soils.
本研究旨在探讨金属动员植物生长有益细菌 Phyllobacterium myrsinacearum RC6b 对 Sedum plumbizincicola 生长和 Cd、Zn 和 Pb 吸收的影响。在收集的一系列耐金属细菌中，P. myrsinacearum RC6b 因其能够动员土壤中高浓度的 Cd、Zn 和 Pb 而被特别选为最有利的金属动员剂。P. myrsinacearum RC6b 对 Cd（350 mg L(-1)）、Zn（1000 mg L(-1)）和 Pb（1200 mg L(-1)）具有高度抗性。此外，P. myrsinacearum RC6b 表现出多种植物生长有益特性，包括 1-氨基环丙烷-1-羧酸脱氨酶、吲哚-3-乙酸、铁载体和不溶性磷酸盐的溶解。接种 P. myrsinacearum RC6b 显著增加了 S. plumbizincicola 的生长和器官金属浓度，但根和茎中的 Pb 浓度较低。结果表明，金属动员 P. myrsinacearum RC6b 可作为一种有效的接种剂，用于改善多金属污染土壤的植物修复。
