Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Gene. 2013 Oct 25;529(2):307-16. doi: 10.1016/j.gene.2013.06.089. Epub 2013 Jul 24.
Verticillium dahliae Kleb. is a phytopathogenic fungus that causes wilt diseases in hundreds of dicotyledonous plant species. Previous research has demonstrated that the secretome plays an important role in the pathogenicity of V. dahliae. In this study, the specific secreted protein gene (VdSSP1) in highly virulent defoliating V. dahliae strain VDG1 was cloned, and considered to be a secreted protein by signal peptide activity assay. VdSSP1 deletion mutants in VDG1 significantly compromised virulence, and the fungal growth decreased in media with pectin and starch as carbon sources. Pathogenicity and carbon utilization were restored upon complementation of the VdSSP1 deletion strains or low virulence non-defoliating strain VDG2, which lacks VdSSP1. It is indicated that the virulence role of VdSSP1 is associated with plant cell wall degradation. In conclusion, our data suggested that VdSSP1 is a secreted protein that is engaged in the pathogenicity of the highly virulent defoliating V. dahliae.
茄镰孢菌（Verticillium dahliae Kleb.）是一种植物病原真菌，可引起数百种双子叶植物的萎蔫病。先前的研究表明，分泌组在茄镰孢菌的致病性中发挥重要作用。在这项研究中，从高度致病的落叶型茄镰孢菌菌株 VDG1 中克隆了特定的分泌蛋白基因（VdSSP1），并通过信号肽活性测定将其视为分泌蛋白。VDG1 中 VdSSP1 缺失突变体的毒力显著降低，并且在果胶和淀粉作为碳源的培养基中真菌生长减少。在补充 VdSSP1 缺失菌株或缺乏 VdSSP1 的低毒非落叶型菌株 VDG2 后，致病性和碳利用得以恢复。这表明 VdSSP1 的毒力作用与植物细胞壁降解有关。总之，我们的数据表明 VdSSP1 是一种参与高度致病的落叶型茄镰孢菌致病性的分泌蛋白。
