College of Animal Science and Technology, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, Heilongjiang 150030, China.
FEBS Lett. 2013 Sep 17;587(18):3032-7. doi: 10.1016/j.febslet.2013.07.033. Epub 2013 Jul 24.
Previous microarray analyses revealed that LMO4 is expressed in 3T3-L1 preadipocytes, however, its roles in adipogenesis are unknown. In the present study, using RT-PCR sequencing and quantitative real-time RT-PCR, we confirmed that LMO4 gene is expressed in 3T3-L1 preadipocytes and its expression peaks at the early stage of 3T3-L1 preadipocyte differentiation. Further analyses showed that LMO4 knockdown decreased the proliferation of 3T3-L1 preadipocytes, and attenuated the differentiation of 3T3-L1 preadipocytes, as evidenced by reduced lipid accumulation and down-regulation of PPARγ gene expression. Collectively, our findings indicate that LMO4 is a novel modulator of adipogenesis.
先前的基因芯片分析表明，LMO4 在 3T3-L1 前脂肪细胞中表达，但其在脂肪生成中的作用尚不清楚。在本研究中，我们通过 RT-PCR 测序和实时定量 RT-PCR 证实 LMO4 基因在 3T3-L1 前脂肪细胞中表达，其表达在 3T3-L1 前脂肪细胞分化的早期达到峰值。进一步的分析表明，LMO4 敲低减少了 3T3-L1 前脂肪细胞的增殖，并减弱了 3T3-L1 前脂肪细胞的分化，表现为脂滴积累减少和 PPARγ 基因表达下调。综上所述，我们的研究结果表明 LMO4 是脂肪生成的一种新型调节因子。
