College of Life Science, Liaocheng University, Liaocheng, China.
Chinese Academy of Geological Sciences, Beijing, China.
Biosci Biotechnol Biochem. 2020 May;84(5):980-988. doi: 10.1080/09168451.2020.1719823. Epub 2020 Jan 27.
Previous RNA-Seq analyses revealed that NAD(P)H steroid dehydrogenase-like () has a different expression during 3T3-L1 differentiation; however, its roles in adipogenesis are unknown. In the present study, using quantitative real-time PCR, we confirmed that knockdown increased the proliferation of 3T3-L1 preadipocytes, but attenuated the differentiation of 3T3-L1 preadipocytes, as evidenced by reduced lipid accumulation and down-regulation of gene expression. Further analyses showed that the expression peak of was at the early stage of 3T3-L1 preadipocytes differentiation and signaling pathway was downregulated in -knockdown 3T3-L1 cells. Collectively, our findings indicate that is a novel modulator of adipogenesis. Moreover, our data provide insight into the complex relationships between sterol sensing, signaling pathway, and in 3T3-L1 cells.
先前的 RNA-Seq 分析表明，NAD(P)H 类固醇脱氢酶样 () 在 3T3-L1 分化过程中表达不同；然而，其在脂肪生成中的作用尚不清楚。在本研究中，我们使用定量实时 PCR 证实了 基因敲低可增加 3T3-L1 前脂肪细胞的增殖，但减弱了 3T3-L1 前脂肪细胞的分化，这表现为脂质积累减少和 基因表达下调。进一步的分析表明，在 3T3-L1 前脂肪细胞分化的早期阶段， 表达峰出现，并且 -knockdown 3T3-L1 细胞中的 信号通路被下调。总之，我们的研究结果表明， 是脂肪生成的一种新型调节剂。此外，我们的数据提供了关于固醇感应、 信号通路和 之间在 3T3-L1 细胞中的复杂关系的深入了解。
