肿瘤坏死因子-α血清水平在炎症性肠病疾病活动评估中的应用

Tumor necrosis factor-alpha serum level in assessment of disease activity in inflammatory bowel diseases.

作者信息

Avdagić Nesina, Babić Nermina, Šeremet Mensura, Delić-Šarac Marina, Drače Zahida, Denjalić Amir, Nakaš-Ićindić Emina

机构信息

1Department of Physiology, School of Medicine, University of Sarajevo, 2Clinical Microbiology, University Clinical Center Sarajevo, 3Clinical Immunology, University Clinical Center Sarajevo, 4 Faculty of Health Studies of Zenica, Bosnia and Herzegovina.

出版信息

Med Glas (Zenica). 2013 Aug;10(2):211-6.

PMID:23892833

Abstract

AIM

To investigate an influence of the concentration of proinflammatory cytokines tumor necrosis factor-alpha (TNF-α) in serum on the activity of inflammatory bowel disease (IBD).

METHODS

The IBD patients of both genders (n=60) were divided in two equal groups, ulcerative colitis (UC) and Crohn's disease (CD). Based on the result of activity index each group was subdivided in two subgroups: active and inactive phase of the disease. Age and gender matched apparently healthy individuals (n=30) involved in the control group. Serum TNF-α concentration was determined by enzyme linked immune-adsorbent assay (ELISA).

RESULTS

The significant difference (Mann-Whitney Test) in serum TNF-α level was found between healthy controls 28.86 pg/ml (28.74 - 29.19 pg/ml) and CD patients (29.47 pg/ml (29.1 - 29.77 pg/ml) (p less than 0.05) and UC patients 29.34 pg/ml (29.14 - 29.71 pg/ ml) (p less than 0.05) respectively. Serum TNF-α level in patients with CD was higher compared to serum TNF-α level in patients with UC, but the difference was not significant (p more than 0,05). There were no significant difference in serum TNF-α concentrations either in CD or UC patients related to the phase of disease activity: active CD 29.53 pg/ml (29.20 - 29.90 pg/ml) vs inactive CD 29.26 pg/ml (29.15 - 29.53 pg/ml); active UC 29.53 pg/ml (29.32 - 29.85 pg/ ml) vs inactive UC 29.26 pg/ml (29.10 - 29.63 pg/ml).

CONCLUSIONS

Since there were no differences in serum TNF-α concentrations related to the disease activity we consider that TNF-α is not an adequate serum biomarker for an assessment of the disease activity in patients with IBD.

摘要

目的

探讨血清中促炎细胞因子肿瘤坏死因子-α（TNF-α）浓度对炎症性肠病（IBD）活动度的影响。

方法

将60例IBD患者分为两组，溃疡性结肠炎（UC）组和克罗恩病（CD）组，每组各30例。根据活动指数结果，每组再分为两个亚组：疾病的活动期和非活动期。选取年龄和性别匹配的30例健康个体作为对照组。采用酶联免疫吸附测定法（ELISA）测定血清TNF-α浓度。

结果

健康对照组血清TNF-α水平为28.86 pg/ml（28.74 - 29.19 pg/ml），CD患者为29.47 pg/ml（29.1 - 29.77 pg/ml），UC患者为29.34 pg/ml（29.14 - 29.71 pg/ml），差异均有统计学意义（Mann-Whitney检验，p均小于0.05）。CD患者血清TNF-α水平高于UC患者，但差异无统计学意义（p大于0.05）。CD或UC患者血清TNF-α浓度在疾病活动期和非活动期之间均无显著差异：活动期CD为29.53 pg/ml（29.20 - 29.90 pg/ml），非活动期CD为29.26 pg/ml（29.15 - 29.53 pg/ml）；活动期UC为29.53 pg/ml（29.32 - 29.85 pg/ml），非活动期UC为29.26 pg/ml（29.10 - 29.63 pg/ml）。