Fukunaga Kazuto, Tsutsumi Hiroshi, Mihara Hisakazu
Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259-B40 Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan.
Biopolymers. 2013 Nov;100(6):731-7. doi: 10.1002/bip.22309.
There is an increasing need for the development of functional artificial extracellular matrices (ECMs) for tissue engineering. Recently, we have successfully designed a self-assembling peptide, named E1Y9, to construct functional ECMs. We describe here an enhancement of abilities of E1Y9 materials to promote cell adhesion and differentiation, using functional peptide sequences derived from natural extracellular matrix proteins. We designed functionalized self-assembling peptides, RGDS-conjugated E1Y9 (E1Y9-RGDS) and IKVAV-conjugated E1Y9 (E1Y9-IKVAV). E1Y9-RGDS and E1Y9-IKVAV formed peptide nanofibers in a similar manner to E1Y9, with β-sheet secondary structures. Surfaces coated with peptide nanofibers displayed the higher bioactivities of E1Y9-RGDS for cell adhesion and E1Y9-IKVAV for cell differentiation than those of E1Y9, with the activities being dependent on the concentrations of the functional peptides. These functionalized peptides will be useful for the construction of functional ECMs in cell and tissue engineering.
组织工程领域对功能性人工细胞外基质（ECM）的需求日益增长。最近，我们成功设计了一种名为E1Y9的自组装肽，用于构建功能性ECM。在此，我们描述了利用源自天然细胞外基质蛋白的功能性肽序列增强E1Y9材料促进细胞黏附和分化的能力。我们设计了功能化的自组装肽，即RGDS偶联的E1Y9（E1Y9-RGDS）和IKVAV偶联的E1Y9（E1Y9-IKVAV）。E1Y9-RGDS和E1Y9-IKVAV与E1Y9以相似的方式形成具有β-折叠二级结构的肽纳米纤维。与E1Y9相比，涂有肽纳米纤维的表面显示出E1Y9-RGDS对细胞黏附以及E1Y9-IKVAV对细胞分化更高的生物活性，且这些活性取决于功能性肽的浓度。这些功能化肽将有助于在细胞和组织工程中构建功能性ECM。
