Fukunaga Kazuto, Tsutsumi Hiroshi, Mihara Hisakazu
Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-Cho, Midori-Ku, Yokohama, 226-8501, Japan.
Biopolymers. 2016 Nov 4;106(4):476-83. doi: 10.1002/bip.22756.
We recently developed a self-assembling peptide, E1Y9, that self-assembles into nanofibers and forms a hydrogel in the presence of Ca(2+) . E1Y9 derivatives conjugated with functional peptide sequences derived from extracellular matrices (ECMs) reportedly self-assemble into peptide nanofibers that enhance cell adhesion and differentiation. In this study, E1Y9/E1Y9-IKVAV-mixed hydrogels were constructed to serve as artificial ECMs that promote cell differentiation. E1Y9 and E1Y9-IKVAV co-assembled into networked nanofibers, and hydrogels with disk and string shapes were formed in response to Ca(2+) treatment. The neuronal differentiation of PC12 cells was facilitated on hydrogels of both shapes that contained the IKVAV motifs. Moreover, long neurites extended along the long axis of the string-shaped gel, suggesting that the structure of hydrogels of this shape can affect cellular orientation. Thus, E1Y9 hydrogels can potentially be used as artificial ECMs with desirable bioactivities and shapes that could be useful in tissue engineering applications. © 2015 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 106: 476-483, 2016.
我们最近开发了一种自组装肽E1Y9，它能自组装成纳米纤维，并在Ca(2+)存在的情况下形成水凝胶。据报道，与源自细胞外基质（ECM）的功能性肽序列共轭的E1Y9衍生物能自组装成增强细胞黏附和分化的肽纳米纤维。在本研究中，构建了E1Y9/E1Y9 - IKVAV混合水凝胶以作为促进细胞分化的人工ECM。E1Y9和E1Y9 - IKVAV共同组装成网络状纳米纤维，并在Ca(2+)处理后形成盘状和串状水凝胶。含有IKVAV基序的两种形状的水凝胶均促进了PC12细胞的神经元分化。此外，长神经突沿着串状凝胶的长轴延伸，表明这种形状的水凝胶结构可影响细胞取向。因此，E1Y9水凝胶有可能用作具有理想生物活性和形状的人工ECM，这在组织工程应用中可能是有用的。© 2015威利期刊公司。生物聚合物（肽科学）106: 476 - 483，2016。
