Jain A V, Schall E D
J Assoc Off Anal Chem. 1975 Jul;58(4):818-21.
A gas-liquid chromatographic (GLC) method is described for the determination of diethylstilbestrol (DES) in molasses-based liquid feed supplements, using dienestrol diacetate as the internal standard. A sample equivalent to 110 mug DES was diluted, acidified with dilute sulfuric acid, extracted with chloroform, and subjected to basic sodium acetate cleanup. The bis-(trimethylsilyl) acetamide derivative was prepared and determined by GLC. Interfering peaks and/or low recoveries were found to be related to emulsions and the procedure incorporates centrifugation to minimize these.
描述了一种气液色谱（GLC）法，用于测定基于糖蜜的液体饲料补充剂中的己烯雌酚（DES），以二乙酸二烯雌酚作为内标。将相当于110微克DES的样品进行稀释，用稀硫酸酸化，用氯仿萃取，并用碱性醋酸钠净化。制备双（三甲基硅基）乙酰胺衍生物并用GLC测定。发现干扰峰和/或回收率低与乳状液有关，该方法采用离心法将这些影响降至最低。
