Einig R G
J Assoc Off Anal Chem. 1976 Jul;59(4):821-4.
A gas-liquid chromatographic method for the determination of diethylstilbestrol (DES) at 2 ppb in mixed feeds and ingredients is presented. The DES is extracted into ethanol, the solvent is evaporated, and the residue is redissolved in chloroform. The extract is purified by washing the chloroform solution with carbonate buffer and transferring the DES to sodium hydroxide. The sodium hydroxide is washed with chloroform, the pH is adjusted to 10.3, and the DES is transferred to chloroform. The extract is derivatized with hepatafluorobutyric anhydride, chromatographed on an SE-30 glass column, and measured with a 3H electron capture detector. The method has been used for routine testing of a wide variety of feeds and ingredients.
本文介绍了一种气相色谱法，用于测定混合饲料和成分中浓度为2 ppb的己烯雌酚（DES）。将DES萃取到乙醇中，蒸发溶剂，残留物再溶解于氯仿中。通过用碳酸盐缓冲液洗涤氯仿溶液并将DES转移至氢氧化钠中来纯化提取物。用氯仿洗涤氢氧化钠，将pH调节至10.3，然后将DES转移至氯仿中。提取物用七氟丁酸酐衍生化，在SE-30玻璃柱上进行色谱分析，并用3H电子捕获检测器进行测定。该方法已用于各种饲料和成分的常规检测。
