Department of Biological Sciences, The George Washington University, Washington, DC 20052.
Genetics. 2013 Oct;195(2):611-9. doi: 10.1534/genetics.113.153106. Epub 2013 Jul 26.
Here we provide the first genome-wide in vivo analysis of the Na+/Ca2+ exchanger family in the model system Caenorhabditis elegans. We source all members of this family within the Caenorhabditis genus and reconstruct their phylogeny across humans and Drosophila melanogaster. Next, we provide a description of the expression pattern for each exchanger gene in C. elegans, revealing a wide expression in a number of tissues and cell types including sensory neurons, interneurons, motor neurons, muscle cells, and intestinal tissue. Finally, we conduct a series of behavioral and functional analyses through mutant characterization in C. elegans. From these data we demonstrate that, similar to mammalian systems, the expression of Na+/Ca2+ exchangers in C. elegans is skewed toward excitable cells, and we propose that C. elegans may be an ideal model system for the study of Na+/Ca2+ exchangers.
在这里，我们在秀丽隐杆线虫这一模式生物系统中对钠离子/钙离子交换家族进行了首次全基因组体内分析。我们在秀丽隐杆线虫属中找到了该家族的所有成员，并在人类和黑腹果蝇中重建了它们的系统发育关系。接下来，我们描述了每个交换基因在秀丽隐杆线虫中的表达模式，揭示了它们在许多组织和细胞类型中的广泛表达，包括感觉神经元、中间神经元、运动神经元、肌肉细胞和肠道组织。最后，我们通过在秀丽隐杆线虫中进行突变特征分析，进行了一系列行为和功能分析。从这些数据中，我们证明了与哺乳动物系统相似，钠离子/钙离子交换在秀丽隐杆线虫中的表达偏向于可兴奋细胞，我们提出秀丽隐杆线虫可能是研究钠离子/钙离子交换的理想模型系统。
