Ingawale Deepa Krushna, Mandlik Satish Karbhari, Kshirsagar Ajay Digambar
Department of Pharmacology, Sinhgad Institute of Pharmaceutical Sciences, Lonavala 410401, India.
J Complement Integr Med. 2013 Jul 23;10:/j/jcim.2013.10.issue-1/jcim-2012-0004/jcim-2012-0004.xml. doi: 10.1515/jcim-2012-0004.
Ethanol extract of Calotropis gigantea flowers (CGFE) was evaluated for its antioxidant and hepatoprotective activity to validate its use in traditional therapeutic indications. This CGFE exhibited significant antioxidant activity (at 20, 40, 60, 80 and 100 µg/ml in vitro) as evidenced by its hydroxyl, nitric oxide and hydrogen peroxide anion radical scavenging activities. This in vitro antioxidant activity was reinforced by a significant hepatoprotection (at 250 and 500 mg/kg dose) by decreasing the activity of serum glutamate oxaloacetate transaminase and serum glutamate pyruvate transaminase. The hepatoprotective activity of the CGFE was comparable with standard drug silymarin (100 mg/kg, p.o.).The results obtained from present study indicate the presence of natural antioxidants and hepatoprotective constituents. Hence, the above finding confirms in vitro antioxidant and hepatoprotective potential of CGFE in mice.
对牛角瓜花乙醇提取物（CGFE）的抗氧化和肝脏保护活性进行了评估，以验证其在传统治疗适应症中的应用。该CGFE表现出显著的抗氧化活性（体外浓度为20、40、60、80和100µg/ml），其对羟基、一氧化氮和过氧化氢阴离子自由基的清除活性证明了这一点。通过降低血清谷氨酸草酰乙酸转氨酶和血清谷氨酸丙酮酸转氨酶的活性，这种体外抗氧化活性在显著的肝脏保护作用（剂量为250和500mg/kg）中得到增强。CGFE的肝脏保护活性与标准药物水飞蓟宾（100mg/kg，口服）相当。本研究获得的结果表明存在天然抗氧化剂和肝脏保护成分。因此，上述发现证实了CGFE在小鼠中的体外抗氧化和肝脏保护潜力。
