Pirlo Russell Kirk, Peng Xiang, Yuan Xiaocong, Gao Bruce Zhi
Department of Bioengineering and Center for Optical Materials Science and Engineering Technologies, Clemson University, Clemson, SC 29634, USA.
Optoelectron Lett. 2008 Sep;4(5):387-390. doi: 10.1007/s11801-008-8059-6.
In this report we illustrate our application of soft lithography-based microfabrication, surface modification, and our unique laser cell-patterning system toward the creation of neuron biochips. We deposited individual forebrain neurons from Day 7 embryonic chicks into two rows of eight in a silicon microstructure aligned over a microelectrode array (MEA). The polydimethylsiloxane (PDMS) membrane with microstructures to confine cells and guide network connectivity was aligned to the electrodes of a MEA. Both the MEA and the PDMS membrane were treated with O plasma, Poly-L-Lysine, and Laminin to aid in cell attachment and survival. The primary advantage of our process is that it is quicker and simpler than previous cell-placement methods and may make highly defined neuronal network biochips more practical.
在本报告中，我们阐述了基于软光刻的微加工、表面改性以及我们独特的激光细胞图案化系统在创建神经元生物芯片方面的应用。我们将来自7日龄胚胎鸡的单个前脑神经元沉积到与微电极阵列（MEA）对齐的硅微结构中两排各八个的位置。带有微结构以限制细胞并引导网络连接的聚二甲基硅氧烷（PDMS）膜与MEA的电极对齐。MEA和PDMS膜都经过氧等离子体、聚-L-赖氨酸和层粘连蛋白处理，以帮助细胞附着和存活。我们方法的主要优点是它比以前的细胞放置方法更快、更简单，并且可能使高度明确的神经网络生物芯片更具实用性。
