Departamento de Ciencias Biologicas, Facultad de Ciencias Biologicas, Universidad Andres Bello, Santiago, Chile.
PLoS One. 2013 Jul 23;8(7):e69983. doi: 10.1371/journal.pone.0069983. Print 2013.
The necessary replacement of fish meal with other protein source in diets of commercially important fish has prompted the study of the effect of the inclusion of different vegetable proteins sources on growth performance and on the gastro-intestinal tract. Currently, soybean meal is the primary protein source as a fish meal replacement because of its low price and high availability. Likewise, it is been documented that the ingestion of soybean meal by several fish species, such as salmonids and carp, triggers a type of intestinal inflammation called enteritis. In this paper, we analyzed the effects of the ingestion of soybean meal and two of its components, soy protein and soy saponin, on zebrafish to establish the basis for using zebrafish larvae as a model for fish nutrition. We took advantage of the existence of different transgenic lines, which allowed us to perform in vivo analysis. Our results indicated that larvae that were feed with soybean meal developed a clear intestinal inflammation as early as two day after beginning the diet. Moreover, we determined that is not the soy protein present in the diet but the soy saponin that is primarily responsible for triggering the immune response. These findings support the use of zebrafish screening assays to identify novel ingredients that would to improved current fish diets or would formulate new ones.
为了在商业上重要的鱼类的饮食中用其他蛋白质来源替代鱼粉，人们研究了添加不同植物蛋白源对生长性能和胃肠道的影响。目前，由于其价格低廉且供应充足，豆粕是替代鱼粉的主要蛋白质来源。同样，有文献记录表明，几种鱼类（如鲑鱼和鲤鱼）摄入豆粕会引发一种称为肠炎的肠道炎症。在本文中，我们分析了摄入豆粕及其两种成分——大豆蛋白和大豆皂甙对斑马鱼的影响，为利用斑马鱼幼虫作为鱼类营养模型奠定了基础。我们利用存在的不同转基因系进行了体内分析。我们的结果表明，从开始摄食豆粕的第二天开始，用豆粕喂养的幼虫就出现了明显的肠道炎症。此外，我们确定引发免疫反应的主要是存在于饮食中的大豆皂甙，而不是大豆蛋白。这些发现支持使用斑马鱼筛选试验来识别可改善当前鱼类饮食或制定新饮食的新成分。
