[日本血吸虫可溶性成虫抗原和虫卵抗原刺激下调节性T细胞的活性]

[Activities of treg cells stimulated by soluble adult worm antigen and egg antigen of Schistosoma japonicum].

作者信息

Dong Xiao-Xiao, Zhang Cui, Yang Xiao-Wei, Li Yong, Chen Xiao-Jun, Xue Xue, Zhang Wei-Wei, Xu Zhi-Peng, Kong Wen-Jun, Zhu Ji-Feng, Zhou Sha, Liu Feng, Su Chuan

机构信息

Department of Pathogen Biology, Nanjing Medical University, Nanjing 210029, China.

出版信息

Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2013 Apr;25(2):146-50.

PMID:23894834

Abstract

OBJECTIVE

To observe and compare the effects of soluble adult worm antigen (SWA) and soluble egg antigen (SEA) of Schistosoma japonicum on the induction of Treg cells and the suppressive activity of Treg cells.

METHOD

Splenocytes were prepared from mice treated with PBS, SWA, and SEA, respectively, and then the proportions of Treg cells and the levels of IL-10 and TGF-beta in Treg cells were determined by FACS. The purified Treg cells from the mice treated as above-mentioned were detected for their immunosuppressive activities by incorporation of [3H] thymidine for the final 16 h of culture.

RESULTS

Compared to SWA, SEA induced the higher proportion of Treg cells with a stronger suppressive activity, which produced the higher levels of IL-10 and TGF-beta (P < 0.05).

CONCLUSION

SEA significantly induces Treg cells and enhances their immunosuppressive activity.

摘要

目的

观察并比较日本血吸虫可溶性成虫抗原（SWA）和可溶性虫卵抗原（SEA）对调节性T细胞（Treg细胞）的诱导作用以及Treg细胞的抑制活性。

方法

分别从经磷酸盐缓冲液（PBS）、SWA和SEA处理的小鼠中制备脾细胞，然后通过流式细胞术（FACS）测定Treg细胞的比例以及Treg细胞中白细胞介素-10（IL-10）和转化生长因子-β（TGF-β）的水平。对上述处理的小鼠纯化的Treg细胞，在培养的最后16小时通过掺入[3H]胸苷来检测其免疫抑制活性。