[日本血吸虫可溶性虫卵抗原和成虫抗原对小鼠效应B细胞分化的影响]

[Effects of soluble egg antigen and adult worm antigen of Schistosoma japonicum on differentiation of effector B cells of mice].

作者信息

Tian Fang, Hu Xue-li, Liu Hao, Wei Hui, Duan Qiu-fang, Qian Li, Yan Hua, Yang Wei-ping

机构信息

Department of Pathogen Biology and Immunology, Yangzhou University Medical College, Yangzhou 225001, China.

出版信息

Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2013 Oct;25(5):488-92.

PMID:24490359

Abstract

OBJECTIVE

To observe the differentiation ability of effector B cells induced by soluble egg antigen (SEA) and soluble adult worm antigen (SWAP) of Schistosoma japonicum.

METHODS

The mouse spleen mononuclear cells and CDl9+ B cells sorted by beadg were stimulated by SEA, SWAP and lipopolysaccharide (LPS), respectively. The ratios of CD19+ IL-6+ cells and CD19+ IFN-gamma+ cells were analyzed by flow cytometry after 72 hours. At the same time, the cytokines IL-6 and IFN-gamma levels in the cultured supernatants were detected by ELISA. The mouse was immunized with the mixture of SEA or SWAP or LPS and the incomplete Freund' s adjuvant for three times, respectively. The mouse spleen mononuclear cells were isolated at the seventh day after the last immunization. The ratios of CD19+ IL-6+ cells and CD19 IFN-gama+ cells were analyzed, and the cytokines IL-6 and IFN-gamma+ levels in the culture supernatants were detected.

RESULTS

The ratio of CD19 IL-6+ cells in spleen mononuclear cells and splenic B cells was significantly increased in the groups stimulated by SEA and LPS (P < 0.05), and the cytokines IL-6 level in the CD193 cells culture supernatants were significantly increased (P < 0.01). Furthermore, the ratio of CD19+ IL-6+ cells and the cytokines IL-6 level were significantly increased in the SEA immunized group (P < 0.01). SWAP could induce a significantly higher ratio of the CD19+ IFN-gamma+ cells in spleen cells, instead of in splenic CD19+ B cells (P < 0.05). The CD19+ IFN-gamma+ cells and the cytokine IFN-gamma level in the culture supernatants in the SWAP immunized group were significantly higher than those in the SEA and PBS immunized groups (P C 6.01).

CONCLUSIONS

SEA preferentially induces the increase of CDl9+ IL-6+ cells in mouse spleen cells; while SWAP preferentially induces the CD19 + IFN-gamma+ cells' production of mouse spleen cells, depending on the effects of other immune cells.

摘要

目的

观察日本血吸虫可溶性虫卵抗原（SEA）和可溶性成虫抗原（SWAP）诱导效应B细胞的分化能力。

方法

分别用SEA、SWAP和脂多糖（LPS）刺激小鼠脾单个核细胞和磁珠分选的CD19⁺ B细胞。72小时后，通过流式细胞术分析CD19⁺ IL-6⁺细胞和CD19⁺ IFN-γ⁺细胞的比例。同时，用ELISA检测培养上清液中细胞因子IL-6和IFN-γ的水平。分别用SEA或SWAP或LPS与不完全弗氏佐剂的混合物免疫小鼠3次。末次免疫后第7天分离小鼠脾单个核细胞，分析CD19⁺ IL-6⁺细胞和CD19 IFN-γ⁺细胞的比例，并检测培养上清液中细胞因子IL-6和IFN-γ的水平。

结果

SEA和LPS刺激组脾单个核细胞和脾B细胞中CD19 IL-6⁺细胞的比例显著增加（P < 0.05），CD19⁺细胞培养上清液中细胞因子IL-6水平显著升高（P < 0.01）。此外，SEA免疫组中CD19⁺ IL-6⁺细胞的比例和细胞因子IL-6水平显著升高（P < 0.01）。SWAP可诱导脾细胞中CD19⁺ IFN-γ⁺细胞的比例显著升高，而不是脾CD19⁺ B细胞中的比例（P < 0.05）。SWAP免疫组培养上清液中的CD19⁺ IFN-γ⁺细胞和细胞因子IFN-γ水平显著高于SEA和PBS免疫组（P < 0.01）。